Strain Fitness in Escherichia coli ECRC100 around OKFHMN_05510

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfocA and pflB are separated by 54 nucleotidespflB and pflA are separated by 191 nucleotides OKFHMN_05505: focA - formate transporter FocA, at 932,059 to 932,916 focA OKFHMN_05510: pflB - formate C-acetyltransferase, at 932,971 to 935,253 pflB OKFHMN_05515: pflA - pyruvate formate lyase 1-activating protein, at 935,445 to 936,185 pflA Position (kb) 932 933 934 935 936Strain fitness (log2 ratio) -2 -1 0 1 2 3at 931.985 kb on - strandat 931.992 kb on - strandat 932.008 kb on + strandat 932.021 kb on + strandat 932.087 kb on + strandat 932.328 kb on + strand, within focAat 932.355 kb on + strand, within focAat 932.355 kb on + strand, within focAat 932.444 kb on + strand, within focAat 932.450 kb on + strand, within focAat 932.470 kb on + strand, within focAat 932.470 kb on + strand, within focAat 932.498 kb on + strand, within focAat 932.507 kb on + strand, within focAat 932.535 kb on + strand, within focAat 932.536 kb on - strand, within focAat 932.582 kb on + strand, within focAat 932.583 kb on - strand, within focAat 932.583 kb on - strand, within focAat 932.583 kb on - strand, within focAat 932.605 kb on + strand, within focAat 932.605 kb on + strand, within focAat 932.606 kb on - strand, within focAat 932.724 kb on - strand, within focAat 932.878 kb on + strandat 932.929 kb on + strandat 932.953 kb on + strandat 932.968 kb on + strandat 932.968 kb on + strandat 932.968 kb on + strandat 932.968 kb on + strandat 933.347 kb on - strand, within pflBat 933.391 kb on + strand, within pflBat 933.532 kb on + strand, within pflBat 933.562 kb on + strand, within pflBat 933.605 kb on + strand, within pflBat 933.749 kb on - strand, within pflBat 933.771 kb on + strand, within pflBat 933.807 kb on + strand, within pflBat 933.829 kb on + strand, within pflBat 934.011 kb on + strand, within pflBat 934.252 kb on - strand, within pflBat 934.468 kb on + strand, within pflBat 934.483 kb on + strand, within pflBat 934.715 kb on + strand, within pflBat 934.807 kb on + strand, within pflBat 935.016 kb on + strand, within pflBat 935.047 kb on + strandat 935.047 kb on + strandat 935.223 kb on + strandat 935.247 kb on + strandat 935.247 kb on + strandat 935.247 kb on + strandat 935.273 kb on + strandat 935.347 kb on - strandat 935.347 kb on - strandat 935.351 kb on - strandat 935.351 kb on - strandat 935.351 kb on - strandat 935.386 kb on - strandat 935.514 kb on - strandat 935.597 kb on + strand, within pflAat 935.643 kb on - strand, within pflAat 935.747 kb on + strand, within pflAat 935.747 kb on + strand, within pflAat 936.093 kb on + strand, within pflAat 936.176 kb on - strandat 936.246 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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931,985 - +3.3
931,992 - +0.7
932,008 + -0.3
932,021 + +1.0
932,087 + +0.9
932,328 + focA OKFHMN_05505 0.31 -0.5
932,355 + focA OKFHMN_05505 0.34 -0.6
932,355 + focA OKFHMN_05505 0.34 +0.7
932,444 + focA OKFHMN_05505 0.45 +0.0
932,450 + focA OKFHMN_05505 0.46 -0.5
932,470 + focA OKFHMN_05505 0.48 +0.1
932,470 + focA OKFHMN_05505 0.48 +0.3
932,498 + focA OKFHMN_05505 0.51 -1.3
932,507 + focA OKFHMN_05505 0.52 -0.1
932,535 + focA OKFHMN_05505 0.55 -0.9
932,536 - focA OKFHMN_05505 0.56 -1.6
932,582 + focA OKFHMN_05505 0.61 -0.0
932,583 - focA OKFHMN_05505 0.61 +0.3
932,583 - focA OKFHMN_05505 0.61 +0.1
932,583 - focA OKFHMN_05505 0.61 -0.7
932,605 + focA OKFHMN_05505 0.64 -0.0
932,605 + focA OKFHMN_05505 0.64 -1.3
932,606 - focA OKFHMN_05505 0.64 +2.3
932,724 - focA OKFHMN_05505 0.78 +1.3
932,878 + +0.6
932,929 + -1.7
932,953 + +0.6
932,968 + +0.8
932,968 + +0.0
932,968 + +1.3
932,968 + +0.3
933,347 - pflB OKFHMN_05510 0.16 +0.0
933,391 + pflB OKFHMN_05510 0.18 -1.1
933,532 + pflB OKFHMN_05510 0.25 -0.0
933,562 + pflB OKFHMN_05510 0.26 +1.3
933,605 + pflB OKFHMN_05510 0.28 +0.4
933,749 - pflB OKFHMN_05510 0.34 +1.3
933,771 + pflB OKFHMN_05510 0.35 -0.4
933,807 + pflB OKFHMN_05510 0.37 +0.4
933,829 + pflB OKFHMN_05510 0.38 +0.3
934,011 + pflB OKFHMN_05510 0.46 +2.3
934,252 - pflB OKFHMN_05510 0.56 +0.4
934,468 + pflB OKFHMN_05510 0.66 +0.7
934,483 + pflB OKFHMN_05510 0.66 +1.3
934,715 + pflB OKFHMN_05510 0.76 +0.7
934,807 + pflB OKFHMN_05510 0.80 -0.1
935,016 + pflB OKFHMN_05510 0.90 -0.6
935,047 + -0.6
935,047 + -0.0
935,223 + -1.0
935,247 + -0.1
935,247 + -0.7
935,247 + +0.3
935,273 + +0.3
935,347 - -0.7
935,347 - -0.6
935,351 - +2.3
935,351 - +2.6
935,351 - +1.6
935,386 - +1.3
935,514 - +0.3
935,597 + pflA OKFHMN_05515 0.21 -1.0
935,643 - pflA OKFHMN_05515 0.27 -0.6
935,747 + pflA OKFHMN_05515 0.41 -0.6
935,747 + pflA OKFHMN_05515 0.41 +0.0
936,093 + pflA OKFHMN_05515 0.87 -0.3
936,176 - +0.3
936,246 - -2.1

Or see this region's nucleotide sequence