Strain Fitness in Escherichia coli ECRC100 around OKFHMN_04020

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfhaC and tnp-IS3 are separated by 180 nucleotidestnp-IS3 and OKFHMN_04020 overlap by 1 nucleotidesOKFHMN_04020 and OKFHMN_04025 are separated by 593 nucleotidesOKFHMN_04025 and tnp-IS3 are separated by 5 nucleotides OKFHMN_04010: fhaC - ShlB/FhaC/HecB family hemolysin secretion/activation protein, at 641,728 to 642,774 fhaC OKFHMN_04015: tnp-IS3 - IS3 family transposase, at 642,955 to 643,281 tnp-IS3 OKFHMN_04020: OKFHMN_04020 - IS3 family transposase, at 643,281 to 644,168 _04020 OKFHMN_04025: OKFHMN_04025 - DUF4222 domain-containing protein, at 644,762 to 644,995 _04025 OKFHMN_04030: tnp-IS3 - IS3 family transposase, at 645,001 to 645,327 tnp-IS3 Position (kb) 643 644 645Strain fitness (log2 ratio) -1 0 1at 642.309 kb on + strand, within fhaCat 642.928 kb on - strandat 644.227 kb on - strandat 644.453 kb on + strandat 644.736 kb on - strandat 644.761 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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642,309 + fhaC OKFHMN_04010 0.55 -0.4
642,928 - -0.2
644,227 - -0.6
644,453 + -0.1
644,736 - -0.3
644,761 + -0.1

Or see this region's nucleotide sequence