Strain Fitness in Escherichia coli ECRC100 around OKFHMN_03015

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_03000 and OKFHMN_03005 overlap by 8 nucleotidesOKFHMN_03005 and OKFHMN_03010 overlap by 4 nucleotidesOKFHMN_03010 and OKFHMN_03015 are separated by 32 nucleotidesOKFHMN_03015 and OKFHMN_03020 are separated by 33 nucleotides OKFHMN_03000: OKFHMN_03000 - Prophage protein, at 479,246 to 479,557 _03000 OKFHMN_03005: OKFHMN_03005 - hypothetical protein, at 479,550 to 479,777 _03005 OKFHMN_03010: OKFHMN_03010 - DNA-binding protein, at 479,774 to 480,055 _03010 OKFHMN_03015: OKFHMN_03015 - DUF1627 domain-containing protein, at 480,088 to 480,804 _03015 OKFHMN_03020: OKFHMN_03020 - replication protein, at 480,838 to 481,500 _03020 Position (kb) 480 481Strain fitness (log2 ratio) -1 0 1at 479.218 kb on + strandat 479.416 kb on - strand, within OKFHMN_03000at 479.617 kb on - strand, within OKFHMN_03005at 479.817 kb on - strand, within OKFHMN_03010at 481.095 kb on - strand, within OKFHMN_03020at 481.168 kb on - strand, within OKFHMN_03020at 481.527 kb on - strandat 481.718 kb on - strandat 481.718 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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479,218 + +0.2
479,416 - OKFHMN_03000 0.54 +0.0
479,617 - OKFHMN_03005 0.29 -0.7
479,817 - OKFHMN_03010 0.15 -0.8
481,095 - OKFHMN_03020 0.39 -0.1
481,168 - OKFHMN_03020 0.50 -0.6
481,527 - +0.4
481,718 - -0.8
481,718 - +0.4

Or see this region's nucleotide sequence