Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02425

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpuuE and puuB are separated by 37 nucleotidespuuB and puuC are separated by 1 nucleotides OKFHMN_02420: puuE - 4-aminobutyrate transaminase, at 370,293 to 371,558 puuE OKFHMN_02425: puuB - gamma-glutamylputrescine oxidase, at 371,596 to 372,876 puuB OKFHMN_02430: puuC - aldehyde dehydrogenase PuuC, at 372,878 to 374,365 puuC Position (kb) 371 372 373Strain fitness (log2 ratio) -1 0 1at 370.710 kb on - strand, within puuEat 370.988 kb on + strand, within puuEat 371.050 kb on + strand, within puuEat 371.055 kb on + strand, within puuEat 371.134 kb on + strand, within puuEat 371.805 kb on - strand, within puuBat 372.167 kb on + strand, within puuBat 372.167 kb on + strand, within puuBat 372.192 kb on - strand, within puuBat 372.220 kb on - strand, within puuBat 372.855 kb on - strandat 373.009 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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370,710 - puuE OKFHMN_02420 0.33 +1.0
370,988 + puuE OKFHMN_02420 0.55 -0.2
371,050 + puuE OKFHMN_02420 0.60 +0.8
371,055 + puuE OKFHMN_02420 0.60 -0.4
371,134 + puuE OKFHMN_02420 0.66 -0.7
371,805 - puuB OKFHMN_02425 0.16 +0.5
372,167 + puuB OKFHMN_02425 0.45 -0.1
372,167 + puuB OKFHMN_02425 0.45 +0.4
372,192 - puuB OKFHMN_02425 0.47 +0.2
372,220 - puuB OKFHMN_02425 0.49 +0.9
372,855 - -0.4
373,009 + +0.5

Or see this region's nucleotide sequence