Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjY and OKFHMN_02275 overlap by 1 nucleotides OKFHMN_02275 and OKFHMN_02280 overlap by 62 nucleotides OKFHMN_02280 and wcaG are separated by 20 nucleotides wcaG and OKFHMN_02290 are separated by 290 nucleotides
OKFHMN_02270: ycjY - Uncharacterized protein YcjY, at 343,704 to 344,624
ycjY
OKFHMN_02275: OKFHMN_02275 - Peptidase-S15 domain-containing protein, at 344,624 to 344,992
_02275
OKFHMN_02280: OKFHMN_02280 - Hydrolase, at 344,931 to 345,554
_02280
OKFHMN_02285: wcaG - NAD-dependent dehydratase, at 345,575 to 346,213
wcaG
OKFHMN_02290: OKFHMN_02290 - Murein peptide amidase A, at 346,504 to 347,232
_02290
OKFHMN_02295: ycjG - L-Ala-D/L-Glu epimerase, at 347,207 to 348,172
ycjG
Position (kb) 345
346
347 Strain fitness (log2 ratio) -1
0
1 at 344.752 kb on + strand, within OKFHMN_02275 at 344.752 kb on + strand, within OKFHMN_02275 at 344.773 kb on + strand, within OKFHMN_02275 at 344.773 kb on + strand, within OKFHMN_02275 at 344.930 kb on + strand, within OKFHMN_02275 at 345.235 kb on - strand, within OKFHMN_02280 at 345.237 kb on + strand, within OKFHMN_02280 at 345.240 kb on - strand, within OKFHMN_02280 at 345.397 kb on - strand, within OKFHMN_02280 at 345.721 kb on + strand, within wcaG at 346.117 kb on - strand, within wcaG at 346.310 kb on - strand at 346.426 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 344,752 + OKFHMN_02275 0.35 +1.1 344,752 + OKFHMN_02275 0.35 +0.3 344,773 + OKFHMN_02275 0.40 -0.4 344,773 + OKFHMN_02275 0.40 +1.1 344,930 + OKFHMN_02275 0.83 +0.0 345,235 - OKFHMN_02280 0.49 -1.4 345,237 + OKFHMN_02280 0.49 +1.1 345,240 - OKFHMN_02280 0.50 +0.3 345,397 - OKFHMN_02280 0.75 -0.2 345,721 + wcaG OKFHMN_02285 0.23 +0.4 346,117 - wcaG OKFHMN_02285 0.85 +1.1 346,310 - -1.4 346,426 - +0.4
Or see this region's nucleotide sequence