Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01765

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydbD and pdxI are separated by 63 nucleotidespdxI and OKFHMN_01770 are separated by 219 nucleotides OKFHMN_01760: ydbD - Uncharacterized protein YdbD, at 257,783 to 260,089 ydbD OKFHMN_01765: pdxI - pyridoxine 4-dehydrogenase, at 260,153 to 261,013 pdxI OKFHMN_01770: OKFHMN_01770 - BigB-like protein, at 261,233 to 263,692 _01770 Position (kb) 260 261 262Strain fitness (log2 ratio) -1 0 1at 260.539 kb on + strand, within pdxIat 261.110 kb on + strandat 261.334 kb on - strandat 261.448 kb on - strandat 261.812 kb on - strand, within OKFHMN_01770at 261.968 kb on - strand, within OKFHMN_01770at 261.968 kb on - strand, within OKFHMN_01770

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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260,539 + pdxI OKFHMN_01765 0.45 -0.8
261,110 + +0.4
261,334 - -0.5
261,448 - -0.6
261,812 - OKFHMN_01770 0.24 -0.6
261,968 - OKFHMN_01770 0.30 -0.5
261,968 - OKFHMN_01770 0.30 +0.7

Or see this region's nucleotide sequence