Strain Fitness in Escherichia coli ECRC100 around OKFHMN_00775

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntint and xis overlap by 11 nucleotidesxis and OKFHMN_00775 are separated by 103 nucleotidesOKFHMN_00775 and OKFHMN_00780 overlap by 4 nucleotidesOKFHMN_00780 and renD overlap by 4 nucleotides OKFHMN_00765: int - Integrase, at 54,662 to 55,804 int OKFHMN_00770: xis - Excisionase, at 55,794 to 56,030 xis OKFHMN_00775: OKFHMN_00775 - replication protein, at 56,134 to 56,958 _00775 OKFHMN_00780: OKFHMN_00780 - Replication protein P, at 56,955 to 57,656 _00780 OKFHMN_00785: renD - Putative protein RenD, at 57,653 to 57,955 renD Position (kb) 56 57Strain fitness (log2 ratio) -3 -2 -1 0 1at 55.193 kb on + strand, within intat 56.101 kb on + strandat 56.522 kb on + strand, within OKFHMN_00775at 56.897 kb on + strandat 57.339 kb on - strand, within OKFHMN_00780at 57.521 kb on + strand, within OKFHMN_00780at 57.626 kb on + strandat 57.653 kb on - strandat 57.694 kb on + strand, within renDat 57.750 kb on + strand, within renD

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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55,193 + int OKFHMN_00765 0.46 +0.6
56,101 + +0.1
56,522 + OKFHMN_00775 0.47 -2.8
56,897 + -0.1
57,339 - OKFHMN_00780 0.55 -1.7
57,521 + OKFHMN_00780 0.81 -0.0
57,626 + +1.1
57,653 - +0.1
57,694 + renD OKFHMN_00785 0.14 -1.2
57,750 + renD OKFHMN_00785 0.32 -1.5

Or see this region's nucleotide sequence