Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28265

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthlyE and umuD are separated by 372 nucleotidesumuD and umuC overlap by 1 nucleotidesumuC and dsbB are separated by 45 nucleotidesdsbB and nhaB are separated by 145 nucleotides OKFHMN_28250: hlyE - hemolysin HlyE, at 5,418,270 to 5,419,181 hlyE OKFHMN_28260: umuD - Protein UmuD, at 5,419,554 to 5,419,973 umuD OKFHMN_28265: umuC - DNA polymerase V catalytic protein, at 5,419,973 to 5,421,241 umuC OKFHMN_28270: dsbB - disulfide bond formation protein DsbB, at 5,421,287 to 5,421,817 dsbB OKFHMN_28275: nhaB - Na(+)/H(+) antiporter NhaB, at 5,421,963 to 5,423,504 nhaB Position (kb) 5419 5420 5421 5422Strain fitness (log2 ratio) -2 -1 0 1 2at 5419.490 kb on + strandat 5419.520 kb on + strandat 5419.707 kb on + strand, within umuDat 5419.708 kb on - strand, within umuDat 5419.805 kb on + strand, within umuDat 5420.363 kb on - strand, within umuCat 5420.371 kb on + strand, within umuCat 5420.776 kb on + strand, within umuCat 5420.879 kb on + strand, within umuCat 5421.361 kb on - strand, within dsbBat 5421.583 kb on - strand, within dsbBat 5421.817 kb on + strandat 5421.943 kb on - strandat 5421.943 kb on - strandat 5421.943 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,419,490 + +0.9
5,419,520 + +0.9
5,419,707 + umuD OKFHMN_28260 0.36 +0.2
5,419,708 - umuD OKFHMN_28260 0.37 +0.2
5,419,805 + umuD OKFHMN_28260 0.60 -1.0
5,420,363 - umuC OKFHMN_28265 0.31 +0.5
5,420,371 + umuC OKFHMN_28265 0.31 +2.5
5,420,776 + umuC OKFHMN_28265 0.63 -0.9
5,420,879 + umuC OKFHMN_28265 0.71 +0.2
5,421,361 - dsbB OKFHMN_28270 0.14 -1.5
5,421,583 - dsbB OKFHMN_28270 0.56 -0.8
5,421,817 + -2.3
5,421,943 - +2.2
5,421,943 - -1.7
5,421,943 - -0.3

Or see this region's nucleotide sequence