Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27710

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttqsA and mdtJ are separated by 411 nucleotidesmdtJ and mdtI overlap by 14 nucleotidesmdtI and ydgD are separated by 38 nucleotidesydgD and ydgU are separated by 99 nucleotides OKFHMN_27700: tqsA - AI-2 transporter TqsA, at 5,335,672 to 5,336,706 tqsA OKFHMN_27705: mdtJ - multidrug/spermidine efflux SMR transporter subunit MdtJ, at 5,337,118 to 5,337,483 mdtJ OKFHMN_27710: mdtI - multidrug/spermidine efflux SMR transporter subunit MdtI, at 5,337,470 to 5,337,799 mdtI OKFHMN_27715: ydgD - Uncharacterized serine protease YdgD, at 5,337,838 to 5,338,659 ydgD OKFHMN_27720: ydgU - Uncharacterized protein YdgU, at 5,338,759 to 5,338,842 ydgU Position (kb) 5337 5338Strain fitness (log2 ratio) -1 0 1 2at 5336.662 kb on - strandat 5336.738 kb on - strandat 5336.903 kb on + strandat 5336.932 kb on + strandat 5337.165 kb on + strand, within mdtJat 5338.074 kb on - strand, within ydgDat 5338.203 kb on + strand, within ydgDat 5338.313 kb on - strand, within ydgDat 5338.653 kb on + strandat 5338.654 kb on - strandat 5338.677 kb on - strandat 5338.705 kb on + strandat 5338.712 kb on + strandat 5338.714 kb on + strandat 5338.754 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,336,662 - +2.5
5,336,738 - +0.4
5,336,903 + -0.6
5,336,932 + -0.4
5,337,165 + mdtJ OKFHMN_27705 0.13 +1.5
5,338,074 - ydgD OKFHMN_27715 0.29 -0.8
5,338,203 + ydgD OKFHMN_27715 0.44 -0.1
5,338,313 - ydgD OKFHMN_27715 0.58 +0.9
5,338,653 + -1.1
5,338,654 - +0.5
5,338,677 - -0.1
5,338,705 + -0.4
5,338,712 + -0.1
5,338,714 + +0.5
5,338,754 + +0.7

Or see this region's nucleotide sequence