Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27535

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnth and rsxE overlap by 1 nucleotidesrsxE and rsxG are separated by 3 nucleotidesrsxG and rsxD are separated by 3 nucleotides OKFHMN_27525: nth - endonuclease III, at 5,298,535 to 5,299,170 nth OKFHMN_27530: rsxE - electron transport complex subunit RsxE, at 5,299,170 to 5,299,865 rsxE OKFHMN_27535: rsxG - electron transport complex subunit RsxG, at 5,299,869 to 5,300,489 rsxG OKFHMN_27540: rsxD - electron transport complex subunit RsxD, at 5,300,493 to 5,301,551 rsxD Position (kb) 5299 5300 5301Strain fitness (log2 ratio) -2 -1 0 1 2at 5298.871 kb on - strand, within nthat 5298.927 kb on - strand, within nthat 5299.030 kb on - strand, within nthat 5299.256 kb on - strand, within rsxEat 5299.372 kb on - strand, within rsxEat 5299.538 kb on + strand, within rsxEat 5299.562 kb on + strand, within rsxEat 5300.142 kb on + strand, within rsxGat 5300.356 kb on - strand, within rsxGat 5300.425 kb on - strand, within rsxGat 5300.623 kb on - strand, within rsxDat 5300.976 kb on - strand, within rsxDat 5300.982 kb on - strand, within rsxDat 5301.018 kb on + strand, within rsxDat 5301.019 kb on - strand, within rsxDat 5301.019 kb on - strand, within rsxDat 5301.019 kb on - strand, within rsxDat 5301.076 kb on - strand, within rsxDat 5301.170 kb on - strand, within rsxDat 5301.304 kb on - strand, within rsxDat 5301.304 kb on - strand, within rsxDat 5301.327 kb on - strand, within rsxD

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,298,871 - nth OKFHMN_27525 0.53 -1.0
5,298,927 - nth OKFHMN_27525 0.62 +0.4
5,299,030 - nth OKFHMN_27525 0.78 +1.8
5,299,256 - rsxE OKFHMN_27530 0.12 +1.5
5,299,372 - rsxE OKFHMN_27530 0.29 +0.6
5,299,538 + rsxE OKFHMN_27530 0.53 -0.5
5,299,562 + rsxE OKFHMN_27530 0.56 -1.6
5,300,142 + rsxG OKFHMN_27535 0.44 -0.4
5,300,356 - rsxG OKFHMN_27535 0.78 +1.5
5,300,425 - rsxG OKFHMN_27535 0.90 -1.8
5,300,623 - rsxD OKFHMN_27540 0.12 +0.2
5,300,976 - rsxD OKFHMN_27540 0.46 +0.5
5,300,982 - rsxD OKFHMN_27540 0.46 -0.7
5,301,018 + rsxD OKFHMN_27540 0.50 -1.1
5,301,019 - rsxD OKFHMN_27540 0.50 -1.5
5,301,019 - rsxD OKFHMN_27540 0.50 -1.6
5,301,019 - rsxD OKFHMN_27540 0.50 +0.6
5,301,076 - rsxD OKFHMN_27540 0.55 -0.3
5,301,170 - rsxD OKFHMN_27540 0.64 -1.8
5,301,304 - rsxD OKFHMN_27540 0.77 -1.8
5,301,304 - rsxD OKFHMN_27540 0.77 -1.1
5,301,327 - rsxD OKFHMN_27540 0.79 -0.5

Or see this region's nucleotide sequence