Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_26000 and OKFHMN_26005 are separated by 29 nucleotides OKFHMN_26005 and xerD are separated by 5 nucleotides xerD and OKFHMN_26015 are separated by 245 nucleotides OKFHMN_26015 and tnp-IS3 overlap by 35 nucleotides
OKFHMN_26000: OKFHMN_26000 - Putative transmembrane protein, at 5,013,943 to 5,014,593
_26000
OKFHMN_26005: OKFHMN_26005 - DUF2511 domain-containing protein, at 5,014,623 to 5,014,970
_26005
OKFHMN_26010: xerD - integrase, at 5,014,976 to 5,015,977
xerD
OKFHMN_26015: OKFHMN_26015 - AMP nucleosidase, at 5,016,223 to 5,016,306
_26015
OKFHMN_26020: tnp-IS3 - IS3 family transposase, at 5,016,272 to 5,017,159
tnp-IS3
Position (kb) 5014
5015
5016 Strain fitness (log2 ratio) -2
-1
0
1 at 5014.209 kb on + strand, within OKFHMN_26000 at 5014.307 kb on + strand, within OKFHMN_26000 at 5014.597 kb on + strand at 5014.622 kb on + strand at 5014.703 kb on + strand, within OKFHMN_26005 at 5014.782 kb on + strand, within OKFHMN_26005 at 5014.871 kb on + strand, within OKFHMN_26005 at 5014.964 kb on + strand at 5015.002 kb on + strand at 5015.002 kb on + strand at 5015.107 kb on + strand, within xerD at 5015.290 kb on + strand, within xerD at 5015.347 kb on + strand, within xerD at 5015.741 kb on + strand, within xerD at 5016.001 kb on + strand at 5016.262 kb on - strand, within OKFHMN_26015
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 5,014,209 + OKFHMN_26000 0.41 -2.0 5,014,307 + OKFHMN_26000 0.56 +0.4 5,014,597 + +0.5 5,014,622 + +0.1 5,014,703 + OKFHMN_26005 0.23 +0.6 5,014,782 + OKFHMN_26005 0.46 +0.7 5,014,871 + OKFHMN_26005 0.71 +0.6 5,014,964 + +0.4 5,015,002 + +0.6 5,015,002 + -0.6 5,015,107 + xerD OKFHMN_26010 0.13 -0.5 5,015,290 + xerD OKFHMN_26010 0.31 -1.1 5,015,347 + xerD OKFHMN_26010 0.37 -1.4 5,015,741 + xerD OKFHMN_26010 0.76 -0.4 5,016,001 + +1.0 5,016,262 - OKFHMN_26015 0.46 -0.9
Or see this region's nucleotide sequence