Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25975

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmarR and OKFHMN_25960 overlap by 4 nucleotidesOKFHMN_25960 and OKFHMN_25965 are separated by 23 nucleotidesOKFHMN_25965 and OKFHMN_25970 are separated by 92 nucleotidesOKFHMN_25970 and OKFHMN_25975 overlap by 4 nucleotidesOKFHMN_25975 and OKFHMN_25980 are separated by 11 nucleotidesOKFHMN_25980 and OKFHMN_25985 are separated by 10 nucleotidesOKFHMN_25985 and OKFHMN_25990 are separated by 21 nucleotides OKFHMN_25955: marR - MarR family transcriptional regulator, at 5,010,997 to 5,011,263 marR OKFHMN_25960: OKFHMN_25960 - hypothetical protein, at 5,011,260 to 5,011,463 _25960 OKFHMN_25965: OKFHMN_25965 - Phage protein, at 5,011,487 to 5,011,903 _25965 OKFHMN_25970: OKFHMN_25970 - Membrane protein, at 5,011,996 to 5,012,109 _25970 OKFHMN_25975: OKFHMN_25975 - DUF4754 domain-containing protein, at 5,012,106 to 5,012,348 _25975 OKFHMN_25980: OKFHMN_25980 - hypothetical protein, at 5,012,360 to 5,012,638 _25980 OKFHMN_25985: OKFHMN_25985 - DUF4761 domain-containing protein, at 5,012,649 to 5,012,999 _25985 OKFHMN_25990: OKFHMN_25990 - DNA-binding protein, at 5,013,021 to 5,013,224 _25990 Position (kb) 5012 5013Strain fitness (log2 ratio) -2 -1 0 1 2at 5011.248 kb on - strandat 5011.278 kb on + strandat 5011.324 kb on + strand, within OKFHMN_25960at 5011.325 kb on - strand, within OKFHMN_25960at 5011.431 kb on - strand, within OKFHMN_25960at 5011.663 kb on - strand, within OKFHMN_25965at 5012.913 kb on - strand, within OKFHMN_25985at 5012.941 kb on - strand, within OKFHMN_25985at 5012.981 kb on + strandat 5013.022 kb on + strandat 5013.137 kb on + strand, within OKFHMN_25990at 5013.138 kb on - strand, within OKFHMN_25990at 5013.140 kb on + strand, within OKFHMN_25990at 5013.140 kb on + strand, within OKFHMN_25990at 5013.150 kb on - strand, within OKFHMN_25990at 5013.166 kb on + strand, within OKFHMN_25990at 5013.180 kb on + strand, within OKFHMN_25990at 5013.271 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,011,248 - -1.7
5,011,278 + +1.0
5,011,324 + OKFHMN_25960 0.31 +0.4
5,011,325 - OKFHMN_25960 0.32 +1.6
5,011,431 - OKFHMN_25960 0.84 -2.1
5,011,663 - OKFHMN_25965 0.42 -1.8
5,012,913 - OKFHMN_25985 0.75 -0.8
5,012,941 - OKFHMN_25985 0.83 +1.6
5,012,981 + +0.1
5,013,022 + +2.6
5,013,137 + OKFHMN_25990 0.57 -0.5
5,013,138 - OKFHMN_25990 0.57 +0.6
5,013,140 + OKFHMN_25990 0.58 +0.2
5,013,140 + OKFHMN_25990 0.58 +0.3
5,013,150 - OKFHMN_25990 0.63 -0.1
5,013,166 + OKFHMN_25990 0.71 -0.3
5,013,180 + OKFHMN_25990 0.78 +0.7
5,013,271 + +0.7

Or see this region's nucleotide sequence