Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25835

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuvrC and pgsA are separated by 56 nucleotidespgsA and Gly_trna are separated by 151 nucleotidesGly_trna and Cys_trna are separated by 53 nucleotidesCys_trna and Leu_trna are separated by 12 nucleotides OKFHMN_25825: uvrC - excinuclease ABC subunit UvrC, at 4,992,128 to 4,993,960 uvrC OKFHMN_25830: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 4,994,017 to 4,994,565 pgsA OKFHMN_25835: Gly_trna - tRNA-Gly(gcc), at 4,994,717 to 4,994,792 _trna OKFHMN_25840: Cys_trna - tRNA-Cys(gca), at 4,994,846 to 4,994,919 _trna OKFHMN_25845: Leu_trna - tRNA-Leu(taa), at 4,994,932 to 4,995,018 _trna Position (kb) 4994 4995Strain fitness (log2 ratio) -1 0 1at 4993.851 kb on - strandat 4994.683 kb on + strandat 4995.461 kb on + strandat 4995.724 kb on + strandat 4995.774 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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4,993,851 - -0.6
4,994,683 + -0.4
4,995,461 + -0.4
4,995,724 + -0.2
4,995,774 + +1.6

Or see this region's nucleotide sequence