Strain Fitness in Escherichia coli ECRC100 around OKFHMN_23050

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfaD and glpC are separated by 192 nucleotidesglpC and glpB overlap by 4 nucleotides OKFHMN_23045: yfaD - ISNCY family transposase, at 4,486,155 to 4,487,081 yfaD OKFHMN_23050: glpC - anaerobic glycerol-3-phosphate dehydrogenase subunit GlpC, at 4,487,274 to 4,488,464 glpC OKFHMN_23055: glpB - glycerol-3-phosphate dehydrogenase subunit GlpB, at 4,488,461 to 4,489,720 glpB Position (kb) 4487 4488 4489Strain fitness (log2 ratio) -2 -1 0 1at 4486.347 kb on - strand, within yfaDat 4486.442 kb on + strand, within yfaDat 4486.442 kb on + strand, within yfaDat 4486.443 kb on - strand, within yfaDat 4486.512 kb on - strand, within yfaDat 4486.563 kb on + strand, within yfaDat 4486.564 kb on - strand, within yfaDat 4486.596 kb on + strand, within yfaDat 4486.596 kb on + strand, within yfaDat 4486.605 kb on - strand, within yfaDat 4486.608 kb on - strand, within yfaDat 4486.850 kb on - strand, within yfaDat 4486.854 kb on - strand, within yfaDat 4486.855 kb on + strand, within yfaDat 4486.931 kb on + strand, within yfaDat 4486.948 kb on - strand, within yfaDat 4486.987 kb on - strand, within yfaDat 4487.030 kb on + strandat 4487.119 kb on + strandat 4487.203 kb on + strandat 4487.204 kb on - strandat 4487.394 kb on + strand, within glpCat 4487.458 kb on + strand, within glpCat 4487.733 kb on - strand, within glpCat 4487.769 kb on + strand, within glpCat 4487.797 kb on - strand, within glpCat 4487.887 kb on - strand, within glpCat 4487.919 kb on + strand, within glpCat 4487.928 kb on - strand, within glpCat 4487.948 kb on - strand, within glpCat 4487.988 kb on - strand, within glpCat 4488.046 kb on + strand, within glpCat 4488.217 kb on + strand, within glpCat 4488.301 kb on + strand, within glpCat 4488.301 kb on + strand, within glpCat 4488.302 kb on - strand, within glpCat 4488.319 kb on + strand, within glpCat 4488.434 kb on + strandat 4488.435 kb on - strandat 4488.507 kb on - strandat 4488.584 kb on - strandat 4488.584 kb on - strandat 4488.686 kb on - strand, within glpBat 4488.687 kb on + strand, within glpBat 4488.688 kb on - strand, within glpBat 4488.688 kb on - strand, within glpBat 4488.857 kb on + strand, within glpBat 4488.858 kb on - strand, within glpBat 4489.110 kb on - strand, within glpBat 4489.110 kb on - strand, within glpBat 4489.132 kb on + strand, within glpBat 4489.209 kb on - strand, within glpBat 4489.304 kb on + strand, within glpBat 4489.304 kb on + strand, within glpBat 4489.404 kb on - strand, within glpBat 4489.439 kb on + strand, within glpB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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4,486,347 - yfaD OKFHMN_23045 0.21 +0.2
4,486,442 + yfaD OKFHMN_23045 0.31 -0.5
4,486,442 + yfaD OKFHMN_23045 0.31 +0.6
4,486,443 - yfaD OKFHMN_23045 0.31 +0.0
4,486,512 - yfaD OKFHMN_23045 0.39 -0.3
4,486,563 + yfaD OKFHMN_23045 0.44 -0.1
4,486,564 - yfaD OKFHMN_23045 0.44 -0.6
4,486,596 + yfaD OKFHMN_23045 0.48 +0.7
4,486,596 + yfaD OKFHMN_23045 0.48 +0.4
4,486,605 - yfaD OKFHMN_23045 0.49 +0.6
4,486,608 - yfaD OKFHMN_23045 0.49 +0.3
4,486,850 - yfaD OKFHMN_23045 0.75 +0.8
4,486,854 - yfaD OKFHMN_23045 0.75 +0.2
4,486,855 + yfaD OKFHMN_23045 0.76 -0.4
4,486,931 + yfaD OKFHMN_23045 0.84 +0.0
4,486,948 - yfaD OKFHMN_23045 0.86 -0.2
4,486,987 - yfaD OKFHMN_23045 0.90 +1.4
4,487,030 + +0.2
4,487,119 + +0.4
4,487,203 + -0.2
4,487,204 - +1.6
4,487,394 + glpC OKFHMN_23050 0.10 +0.9
4,487,458 + glpC OKFHMN_23050 0.15 -2.0
4,487,733 - glpC OKFHMN_23050 0.39 +0.2
4,487,769 + glpC OKFHMN_23050 0.42 +1.4
4,487,797 - glpC OKFHMN_23050 0.44 +0.8
4,487,887 - glpC OKFHMN_23050 0.51 +1.4
4,487,919 + glpC OKFHMN_23050 0.54 -0.1
4,487,928 - glpC OKFHMN_23050 0.55 -0.5
4,487,948 - glpC OKFHMN_23050 0.57 -0.7
4,487,988 - glpC OKFHMN_23050 0.60 -0.5
4,488,046 + glpC OKFHMN_23050 0.65 -0.3
4,488,217 + glpC OKFHMN_23050 0.79 +0.2
4,488,301 + glpC OKFHMN_23050 0.86 +1.0
4,488,301 + glpC OKFHMN_23050 0.86 -0.1
4,488,302 - glpC OKFHMN_23050 0.86 +0.2
4,488,319 + glpC OKFHMN_23050 0.88 -1.5
4,488,434 + -0.5
4,488,435 - +0.7
4,488,507 - -0.0
4,488,584 - +0.0
4,488,584 - +0.3
4,488,686 - glpB OKFHMN_23055 0.18 +0.2
4,488,687 + glpB OKFHMN_23055 0.18 -0.0
4,488,688 - glpB OKFHMN_23055 0.18 +1.0
4,488,688 - glpB OKFHMN_23055 0.18 -0.7
4,488,857 + glpB OKFHMN_23055 0.31 -1.7
4,488,858 - glpB OKFHMN_23055 0.32 +0.8
4,489,110 - glpB OKFHMN_23055 0.52 -0.2
4,489,110 - glpB OKFHMN_23055 0.52 -0.5
4,489,132 + glpB OKFHMN_23055 0.53 +0.4
4,489,209 - glpB OKFHMN_23055 0.59 +1.6
4,489,304 + glpB OKFHMN_23055 0.67 +0.7
4,489,304 + glpB OKFHMN_23055 0.67 +0.5
4,489,404 - glpB OKFHMN_23055 0.75 +0.5
4,489,439 + glpB OKFHMN_23055 0.78 -0.7

Or see this region's nucleotide sequence