Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20960

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_20950 and alpA are separated by 99 nucleotidesalpA and xerD are separated by 322 nucleotidesxerD and OKFHMN_20965 are separated by 1 nucleotides OKFHMN_20950: OKFHMN_20950 - Uncharacterized conserved protein, contains a C-terminal beta-barrel porin domain, at 4,071,688 to 4,075,629 _20950 OKFHMN_20955: alpA - dipicolinate synthase, at 4,075,729 to 4,075,935 alpA OKFHMN_20960: xerD - site-specific integrase, at 4,076,258 to 4,077,463 xerD OKFHMN_20965: OKFHMN_20965 - integrase, at 4,077,465 to 4,078,778 _20965 Position (kb) 4076 4077 4078Strain fitness (log2 ratio) -1 0 1 2at 4075.415 kb on + strandat 4075.457 kb on + strandat 4075.502 kb on - strandat 4075.502 kb on - strandat 4075.516 kb on - strandat 4075.643 kb on + strandat 4075.729 kb on - strandat 4076.054 kb on + strandat 4076.419 kb on + strand, within xerDat 4076.620 kb on - strand, within xerDat 4076.708 kb on - strand, within xerDat 4076.871 kb on + strand, within xerDat 4077.015 kb on + strand, within xerDat 4077.081 kb on + strand, within xerDat 4077.230 kb on + strand, within xerDat 4078.259 kb on + strand, within OKFHMN_20965

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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4,075,415 + +2.3
4,075,457 + +1.5
4,075,502 - -0.6
4,075,502 - +0.7
4,075,516 - +0.6
4,075,643 + +1.3
4,075,729 - +0.1
4,076,054 + -0.8
4,076,419 + xerD OKFHMN_20960 0.13 +0.8
4,076,620 - xerD OKFHMN_20960 0.30 +0.1
4,076,708 - xerD OKFHMN_20960 0.37 +1.8
4,076,871 + xerD OKFHMN_20960 0.51 -0.2
4,077,015 + xerD OKFHMN_20960 0.63 -0.4
4,077,081 + xerD OKFHMN_20960 0.68 +1.3
4,077,230 + xerD OKFHMN_20960 0.81 +1.4
4,078,259 + OKFHMN_20965 0.60 -0.6

Or see this region's nucleotide sequence