Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20325

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygcU and ygcS are separated by 21 nucleotidesygcS and ygcS overlap by 4 nucleotidesygcS and ygcR overlap by 23 nucleotidesygcR and fixB overlap by 4 nucleotides OKFHMN_20320: ygcU - Uncharacterized FAD-linked oxidoreductase YgcU, at 3,956,854 to 3,958,308 ygcU OKFHMN_20325: ygcS - Inner membrane metabolite transport protein YgcS, at 3,958,330 to 3,959,535 ygcS OKFHMN_20330: ygcS - Inner membrane metabolite transport protein YgcS, at 3,959,532 to 3,959,738 ygcS OKFHMN_20335: ygcR - Putative electron transfer flavoprotein subunit YgcR, at 3,959,716 to 3,960,495 ygcR OKFHMN_20340: fixB - electron transfer flavoprotein, at 3,960,492 to 3,961,061 fixB Position (kb) 3958 3959 3960Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3957.497 kb on - strand, within ygcUat 3957.553 kb on + strand, within ygcUat 3957.559 kb on + strand, within ygcUat 3957.941 kb on - strand, within ygcUat 3958.020 kb on + strand, within ygcUat 3958.020 kb on + strand, within ygcUat 3958.021 kb on - strand, within ygcUat 3958.021 kb on - strand, within ygcUat 3958.021 kb on - strand, within ygcUat 3958.021 kb on - strand, within ygcUat 3958.045 kb on + strand, within ygcUat 3958.045 kb on + strand, within ygcUat 3958.045 kb on + strand, within ygcUat 3958.060 kb on + strand, within ygcUat 3958.077 kb on + strand, within ygcUat 3958.102 kb on + strand, within ygcUat 3958.103 kb on - strand, within ygcUat 3958.272 kb on + strandat 3958.287 kb on + strandat 3958.287 kb on + strandat 3958.288 kb on - strandat 3958.498 kb on + strand, within ygcSat 3958.499 kb on - strand, within ygcSat 3958.499 kb on - strand, within ygcSat 3958.527 kb on + strand, within ygcSat 3958.775 kb on - strand, within ygcSat 3958.781 kb on + strand, within ygcSat 3958.781 kb on + strand, within ygcSat 3958.862 kb on + strand, within ygcSat 3958.867 kb on + strand, within ygcSat 3958.867 kb on + strand, within ygcSat 3958.868 kb on - strand, within ygcSat 3958.868 kb on - strand, within ygcSat 3958.868 kb on - strand, within ygcSat 3958.966 kb on - strand, within ygcSat 3958.966 kb on - strand, within ygcSat 3959.104 kb on - strand, within ygcSat 3959.229 kb on + strand, within ygcSat 3959.229 kb on + strand, within ygcSat 3959.229 kb on + strand, within ygcSat 3959.268 kb on + strand, within ygcSat 3959.304 kb on - strand, within ygcSat 3959.375 kb on + strand, within ygcSat 3959.456 kb on + strandat 3959.608 kb on + strand, within ygcSat 3959.647 kb on + strand, within ygcSat 3959.647 kb on + strand, within ygcSat 3959.982 kb on - strand, within ygcRat 3960.210 kb on + strand, within ygcRat 3960.210 kb on + strand, within ygcRat 3960.458 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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3,957,497 - ygcU OKFHMN_20320 0.44 +0.6
3,957,553 + ygcU OKFHMN_20320 0.48 +0.9
3,957,559 + ygcU OKFHMN_20320 0.48 +1.2
3,957,941 - ygcU OKFHMN_20320 0.75 +0.0
3,958,020 + ygcU OKFHMN_20320 0.80 -0.9
3,958,020 + ygcU OKFHMN_20320 0.80 -0.3
3,958,021 - ygcU OKFHMN_20320 0.80 +1.0
3,958,021 - ygcU OKFHMN_20320 0.80 -0.2
3,958,021 - ygcU OKFHMN_20320 0.80 +1.1
3,958,021 - ygcU OKFHMN_20320 0.80 +0.1
3,958,045 + ygcU OKFHMN_20320 0.82 -0.0
3,958,045 + ygcU OKFHMN_20320 0.82 +0.1
3,958,045 + ygcU OKFHMN_20320 0.82 -0.4
3,958,060 + ygcU OKFHMN_20320 0.83 +0.3
3,958,077 + ygcU OKFHMN_20320 0.84 -0.1
3,958,102 + ygcU OKFHMN_20320 0.86 -0.1
3,958,103 - ygcU OKFHMN_20320 0.86 -0.9
3,958,272 + -0.2
3,958,287 + -0.5
3,958,287 + +1.2
3,958,288 - +0.5
3,958,498 + ygcS OKFHMN_20325 0.14 +0.4
3,958,499 - ygcS OKFHMN_20325 0.14 -1.3
3,958,499 - ygcS OKFHMN_20325 0.14 -0.5
3,958,527 + ygcS OKFHMN_20325 0.16 +0.1
3,958,775 - ygcS OKFHMN_20325 0.37 -1.2
3,958,781 + ygcS OKFHMN_20325 0.37 -1.7
3,958,781 + ygcS OKFHMN_20325 0.37 +1.0
3,958,862 + ygcS OKFHMN_20325 0.44 -0.9
3,958,867 + ygcS OKFHMN_20325 0.45 -0.0
3,958,867 + ygcS OKFHMN_20325 0.45 +0.2
3,958,868 - ygcS OKFHMN_20325 0.45 -2.9
3,958,868 - ygcS OKFHMN_20325 0.45 +1.8
3,958,868 - ygcS OKFHMN_20325 0.45 +0.6
3,958,966 - ygcS OKFHMN_20325 0.53 +0.9
3,958,966 - ygcS OKFHMN_20325 0.53 +0.3
3,959,104 - ygcS OKFHMN_20325 0.64 -0.2
3,959,229 + ygcS OKFHMN_20325 0.75 +0.2
3,959,229 + ygcS OKFHMN_20325 0.75 -0.1
3,959,229 + ygcS OKFHMN_20325 0.75 +0.3
3,959,268 + ygcS OKFHMN_20325 0.78 -0.5
3,959,304 - ygcS OKFHMN_20325 0.81 -0.4
3,959,375 + ygcS OKFHMN_20325 0.87 +0.1
3,959,456 + -1.4
3,959,608 + ygcS OKFHMN_20330 0.37 -0.1
3,959,647 + ygcS OKFHMN_20330 0.56 +0.1
3,959,647 + ygcS OKFHMN_20330 0.56 -0.9
3,959,982 - ygcR OKFHMN_20335 0.34 +1.8
3,960,210 + ygcR OKFHMN_20335 0.63 +1.4
3,960,210 + ygcR OKFHMN_20335 0.63 -0.4
3,960,458 + +0.7

Or see this region's nucleotide sequence