Strain Fitness in Escherichia coli ECRC100 around OKFHMN_19785

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_19775 and fliN overlap by 41 nucleotidesfliN and escR overlap by 11 nucleotidesescR and OKFHMN_19790 are separated by 10 nucleotidesOKFHMN_19790 and sctT are separated by 1 nucleotidessctT and sctT are separated by 78 nucleotides OKFHMN_19775: OKFHMN_19775 - type III secretion protein, at 3,849,216 to 3,849,785 _19775 OKFHMN_19780: fliN - YscQ/HrcQ family type III secretion apparatus protein, at 3,849,745 to 3,850,731 fliN OKFHMN_19785: escR - EscR/YscR/HrcR family type III secretion system export apparatus protein, at 3,850,721 to 3,851,386 escR OKFHMN_19790: OKFHMN_19790 - EscS/YscS/HrcS family type III secretion system export apparatus protein, at 3,851,397 to 3,851,657 _19790 OKFHMN_19795: sctT - type III secretion system export apparatus subunit SctT, at 3,851,659 to 3,852,111 sctT OKFHMN_19800: sctT - type III secretion system export apparatus subunit SctT, at 3,852,190 to 3,852,426 sctT Position (kb) 3850 3851 3852Strain fitness (log2 ratio) -1 0 1at 3849.875 kb on + strand, within fliNat 3850.408 kb on + strand, within fliNat 3851.217 kb on + strand, within escRat 3851.258 kb on - strand, within escRat 3851.287 kb on + strand, within escRat 3851.672 kb on + strandat 3851.801 kb on + strand, within sctTat 3851.919 kb on - strand, within sctTat 3852.271 kb on + strand, within sctT

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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3,849,875 + fliN OKFHMN_19780 0.13 +0.1
3,850,408 + fliN OKFHMN_19780 0.67 +0.2
3,851,217 + escR OKFHMN_19785 0.74 +0.3
3,851,258 - escR OKFHMN_19785 0.81 -0.3
3,851,287 + escR OKFHMN_19785 0.85 +0.3
3,851,672 + -0.3
3,851,801 + sctT OKFHMN_19795 0.31 -0.4
3,851,919 - sctT OKFHMN_19795 0.57 +0.3
3,852,271 + sctT OKFHMN_19800 0.34 +0.7

Or see this region's nucleotide sequence