Strain Fitness in Escherichia coli ECRC100 around OKFHMN_18645

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnfeF and Ile2_trna are separated by 53 nucleotidesIle2_trna and mug are separated by 124 nucleotidesmug and rpoD are separated by 78 nucleotides OKFHMN_18640: nfeF - NADPH-dependent ferric chelate reductase, at 3,620,373 to 3,621,137 nfeF OKFHMN_18645: Ile2_trna - tRNA-Ile2(cat), at 3,621,191 to 3,621,266 _trna OKFHMN_18650: mug - G/U mismatch-specific DNA glycosylase, at 3,621,391 to 3,621,897 mug OKFHMN_18655: rpoD - RNA polymerase sigma factor RpoD, at 3,621,976 to 3,623,817 rpoD Position (kb) 3621 3622Strain fitness (log2 ratio) -2 -1 0 1at 3620.226 kb on + strandat 3620.227 kb on - strandat 3620.227 kb on - strandat 3620.227 kb on - strandat 3620.267 kb on - strandat 3620.267 kb on - strandat 3620.267 kb on - strandat 3620.351 kb on + strandat 3620.440 kb on + strandat 3620.453 kb on + strand, within nfeFat 3620.508 kb on - strand, within nfeFat 3620.635 kb on - strand, within nfeFat 3620.635 kb on - strand, within nfeFat 3620.637 kb on - strand, within nfeFat 3620.637 kb on - strand, within nfeFat 3620.656 kb on + strand, within nfeFat 3620.657 kb on - strand, within nfeFat 3620.657 kb on - strand, within nfeFat 3620.739 kb on - strand, within nfeFat 3620.802 kb on + strand, within nfeFat 3620.802 kb on + strand, within nfeFat 3620.803 kb on - strand, within nfeFat 3620.803 kb on - strand, within nfeFat 3620.882 kb on + strand, within nfeFat 3620.882 kb on + strand, within nfeFat 3620.882 kb on + strand, within nfeFat 3620.945 kb on + strand, within nfeFat 3620.980 kb on + strand, within nfeFat 3621.021 kb on + strand, within nfeFat 3621.021 kb on + strand, within nfeFat 3621.186 kb on - strandat 3621.186 kb on - strandat 3621.188 kb on - strandat 3621.258 kb on + strand, within Ile2_trnaat 3621.280 kb on - strandat 3621.287 kb on - strandat 3621.331 kb on + strandat 3621.359 kb on - strandat 3621.386 kb on + strandat 3621.386 kb on + strandat 3621.386 kb on + strandat 3621.387 kb on - strandat 3621.387 kb on - strandat 3621.387 kb on - strandat 3621.387 kb on - strandat 3621.403 kb on - strandat 3621.512 kb on + strand, within mugat 3621.512 kb on + strand, within mugat 3621.512 kb on + strand, within mugat 3621.512 kb on + strand, within mugat 3621.513 kb on - strand, within mugat 3621.513 kb on - strand, within mugat 3621.660 kb on + strand, within mugat 3621.724 kb on + strand, within mugat 3621.724 kb on + strand, within mugat 3621.978 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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3,620,226 + -0.4
3,620,227 - -1.5
3,620,227 - -1.2
3,620,227 - -0.6
3,620,267 - -0.4
3,620,267 - -0.5
3,620,267 - +0.0
3,620,351 + -0.0
3,620,440 + +0.2
3,620,453 + nfeF OKFHMN_18640 0.10 -0.3
3,620,508 - nfeF OKFHMN_18640 0.18 -0.4
3,620,635 - nfeF OKFHMN_18640 0.34 +0.2
3,620,635 - nfeF OKFHMN_18640 0.34 +0.1
3,620,637 - nfeF OKFHMN_18640 0.35 -0.3
3,620,637 - nfeF OKFHMN_18640 0.35 +0.0
3,620,656 + nfeF OKFHMN_18640 0.37 +1.1
3,620,657 - nfeF OKFHMN_18640 0.37 +0.6
3,620,657 - nfeF OKFHMN_18640 0.37 +0.4
3,620,739 - nfeF OKFHMN_18640 0.48 -0.3
3,620,802 + nfeF OKFHMN_18640 0.56 +0.4
3,620,802 + nfeF OKFHMN_18640 0.56 -1.0
3,620,803 - nfeF OKFHMN_18640 0.56 -0.8
3,620,803 - nfeF OKFHMN_18640 0.56 -0.4
3,620,882 + nfeF OKFHMN_18640 0.67 +0.2
3,620,882 + nfeF OKFHMN_18640 0.67 -0.9
3,620,882 + nfeF OKFHMN_18640 0.67 +0.4
3,620,945 + nfeF OKFHMN_18640 0.75 -0.5
3,620,980 + nfeF OKFHMN_18640 0.79 -0.9
3,621,021 + nfeF OKFHMN_18640 0.85 +0.7
3,621,021 + nfeF OKFHMN_18640 0.85 -1.3
3,621,186 - -0.1
3,621,186 - -0.5
3,621,188 - -0.1
3,621,258 + Ile2_trna OKFHMN_18645 0.88 -0.0
3,621,280 - -0.8
3,621,287 - +1.0
3,621,331 + +0.5
3,621,359 - +0.1
3,621,386 + +0.1
3,621,386 + -0.0
3,621,386 + +0.3
3,621,387 - +0.5
3,621,387 - -0.0
3,621,387 - -0.7
3,621,387 - +0.3
3,621,403 - +0.4
3,621,512 + mug OKFHMN_18650 0.24 -0.5
3,621,512 + mug OKFHMN_18650 0.24 -0.8
3,621,512 + mug OKFHMN_18650 0.24 +0.5
3,621,512 + mug OKFHMN_18650 0.24 -0.0
3,621,513 - mug OKFHMN_18650 0.24 +0.5
3,621,513 - mug OKFHMN_18650 0.24 -1.7
3,621,660 + mug OKFHMN_18650 0.53 -0.2
3,621,724 + mug OKFHMN_18650 0.66 +0.3
3,621,724 + mug OKFHMN_18650 0.66 +0.3
3,621,978 - -2.4

Or see this region's nucleotide sequence