Strain Fitness in Escherichia coli ECRC100 around OKFHMN_18015

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmurA and sfsB are separated by 47 nucleotidessfsB and ispB are separated by 228 nucleotidesispB and rplU are separated by 258 nucleotidesrplU and rpmA are separated by 20 nucleotidesrpmA and yhbE are separated by 126 nucleotides OKFHMN_18005: murA - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 3,497,549 to 3,498,808 murA OKFHMN_18010: sfsB - DNA-binding transcriptional regulator SfsB, at 3,498,856 to 3,499,134 sfsB OKFHMN_18015: ispB - octaprenyl diphosphate synthase, at 3,499,363 to 3,500,334 ispB OKFHMN_18020: rplU - 50S ribosomal protein L21, at 3,500,593 to 3,500,904 rplU OKFHMN_18025: rpmA - 50S ribosomal protein L27, at 3,500,925 to 3,501,182 rpmA OKFHMN_18030: yhbE - Uncharacterized inner membrane transporter YhbE, at 3,501,309 to 3,502,274 yhbE Position (kb) 3499 3500 3501Strain fitness (log2 ratio) -2 -1 0 1at 3498.870 kb on + strandat 3498.875 kb on + strandat 3498.876 kb on - strandat 3498.929 kb on + strand, within sfsBat 3498.946 kb on - strand, within sfsBat 3498.946 kb on - strand, within sfsBat 3498.946 kb on - strand, within sfsBat 3498.979 kb on - strand, within sfsBat 3498.979 kb on - strand, within sfsBat 3499.126 kb on - strandat 3499.126 kb on - strandat 3499.149 kb on + strandat 3499.149 kb on + strandat 3499.195 kb on + strandat 3499.233 kb on - strandat 3499.257 kb on - strandat 3499.266 kb on + strandat 3499.267 kb on - strandat 3499.267 kb on - strandat 3499.267 kb on - strandat 3499.289 kb on + strandat 3499.306 kb on - strandat 3500.368 kb on - strandat 3500.388 kb on - strandat 3500.407 kb on - strandat 3500.407 kb on - strandat 3500.407 kb on - strandat 3500.409 kb on - strandat 3500.409 kb on - strandat 3500.451 kb on + strandat 3500.451 kb on + strandat 3500.451 kb on + strandat 3500.486 kb on + strandat 3500.487 kb on - strandat 3501.237 kb on + strandat 3501.303 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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3,498,870 + -0.0
3,498,875 + -0.4
3,498,876 - +0.4
3,498,929 + sfsB OKFHMN_18010 0.26 -1.0
3,498,946 - sfsB OKFHMN_18010 0.32 -0.4
3,498,946 - sfsB OKFHMN_18010 0.32 -0.2
3,498,946 - sfsB OKFHMN_18010 0.32 +0.3
3,498,979 - sfsB OKFHMN_18010 0.44 -0.1
3,498,979 - sfsB OKFHMN_18010 0.44 -1.5
3,499,126 - -0.4
3,499,126 - -0.6
3,499,149 + -0.4
3,499,149 + +0.6
3,499,195 + +0.2
3,499,233 - -0.3
3,499,257 - +0.1
3,499,266 + -0.1
3,499,267 - -0.0
3,499,267 - +0.6
3,499,267 - -0.5
3,499,289 + +0.0
3,499,306 - -0.3
3,500,368 - -2.0
3,500,388 - -0.5
3,500,407 - -2.5
3,500,407 - -0.4
3,500,407 - -0.8
3,500,409 - +0.6
3,500,409 - -0.3
3,500,451 + -0.3
3,500,451 + -0.7
3,500,451 + -1.7
3,500,486 + +0.8
3,500,487 - +1.4
3,501,237 + -0.2
3,501,303 + +1.0

Or see this region's nucleotide sequence