Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gadE and hdeD are separated by 798 nucleotides hdeD and hdeA are separated by 254 nucleotides hdeA and hdeB are separated by 103 nucleotides
OKFHMN_16295: gadE - acid resistance transcriptional activator GadE, at 3,174,055 to 3,174,582
gadE
OKFHMN_16300: hdeD - acid-resistance protein HdeD, at 3,175,381 to 3,175,953
hdeD
OKFHMN_16305: hdeA - acid-activated periplasmic chaperone HdeA, at 3,176,208 to 3,176,540
hdeA
OKFHMN_16310: hdeB - acid-activated periplasmic chaperone HdeB, at 3,176,644 to 3,176,982
hdeB
Position (kb)
3175
3176 Strain fitness (log2 ratio)
-1
0
1
2 at 3174.445 kb on - strand, within gadE at 3174.582 kb on + strand at 3174.664 kb on - strand at 3174.699 kb on + strand at 3174.703 kb on - strand at 3174.703 kb on - strand at 3174.990 kb on - strand at 3175.018 kb on + strand at 3175.089 kb on + strand at 3175.093 kb on + strand at 3175.300 kb on + strand at 3175.378 kb on + strand at 3175.382 kb on + strand at 3175.574 kb on + strand, within hdeD at 3175.623 kb on + strand, within hdeD at 3175.731 kb on + strand, within hdeD at 3175.783 kb on + strand, within hdeD at 3175.787 kb on - strand, within hdeD at 3175.787 kb on - strand, within hdeD at 3175.795 kb on + strand, within hdeD at 3175.796 kb on - strand, within hdeD at 3175.796 kb on - strand, within hdeD at 3175.798 kb on + strand, within hdeD at 3175.939 kb on - strand at 3175.943 kb on - strand at 3175.944 kb on + strand at 3175.944 kb on + strand at 3175.946 kb on + strand at 3175.947 kb on - strand at 3175.947 kb on - strand at 3175.947 kb on - strand at 3175.949 kb on - strand at 3175.949 kb on - strand at 3175.949 kb on - strand at 3175.953 kb on + strand at 3175.954 kb on - strand at 3175.954 kb on - strand at 3175.973 kb on - strand at 3176.191 kb on + strand at 3176.192 kb on - strand at 3176.400 kb on - strand, within hdeA at 3176.419 kb on + strand, within hdeA at 3176.468 kb on + strand, within hdeA at 3176.610 kb on + strand at 3176.625 kb on + strand at 3176.845 kb on + strand, within hdeB at 3176.846 kb on - strand, within hdeB at 3176.847 kb on + strand, within hdeB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 3,174,445 - gadE OKFHMN_16295 0.74 +0.2 3,174,582 + +0.6 3,174,664 - -0.3 3,174,699 + -1.7 3,174,703 - -0.7 3,174,703 - -0.2 3,174,990 - +0.1 3,175,018 + -0.2 3,175,089 + -1.0 3,175,093 + +2.0 3,175,300 + +0.6 3,175,378 + +0.3 3,175,382 + +0.5 3,175,574 + hdeD OKFHMN_16300 0.34 +0.2 3,175,623 + hdeD OKFHMN_16300 0.42 -0.8 3,175,731 + hdeD OKFHMN_16300 0.61 +0.2 3,175,783 + hdeD OKFHMN_16300 0.70 +0.3 3,175,787 - hdeD OKFHMN_16300 0.71 -1.2 3,175,787 - hdeD OKFHMN_16300 0.71 +1.0 3,175,795 + hdeD OKFHMN_16300 0.72 +0.3 3,175,796 - hdeD OKFHMN_16300 0.72 -0.3 3,175,796 - hdeD OKFHMN_16300 0.72 +0.0 3,175,798 + hdeD OKFHMN_16300 0.73 +0.3 3,175,939 - -0.3 3,175,943 - -0.1 3,175,944 + -0.5 3,175,944 + -0.5 3,175,946 + -0.8 3,175,947 - +0.7 3,175,947 - +0.6 3,175,947 - +0.2 3,175,949 - -0.3 3,175,949 - -0.1 3,175,949 - -0.4 3,175,953 + +0.5 3,175,954 - -0.2 3,175,954 - -0.4 3,175,973 - -0.4 3,176,191 + -1.4 3,176,192 - +2.0 3,176,400 - hdeA OKFHMN_16305 0.58 -0.1 3,176,419 + hdeA OKFHMN_16305 0.63 -1.0 3,176,468 + hdeA OKFHMN_16305 0.78 +0.9 3,176,610 + +0.3 3,176,625 + +0.2 3,176,845 + hdeB OKFHMN_16310 0.59 +1.2 3,176,846 - hdeB OKFHMN_16310 0.60 -0.1 3,176,847 + hdeB OKFHMN_16310 0.60 +0.9
Or see this region's nucleotide sequence