Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15355

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescJ and escI are separated by 2 nucleotidesescI and espZ are separated by 83 nucleotidesespZ and cesL are separated by 184 nucleotidescesL and escV overlap by 4 nucleotides OKFHMN_15340: escJ - type III secretion system LEE inner membrane ring protein EscJ, at 2,964,164 to 2,964,736 escJ OKFHMN_15345: escI - Type III secretion system inner rod component EscI, at 2,964,739 to 2,965,116 escI OKFHMN_15350: espZ - type III secretion system LEE cytoprotective effector EspZ, at 2,965,200 to 2,965,499 espZ OKFHMN_15355: cesL - type III secretion system LEE chaperone CesL, at 2,965,684 to 2,966,037 cesL OKFHMN_15360: escV - type III secretion system LEE export apparatus protein EscV, at 2,966,034 to 2,968,061 escV Position (kb) 2965 2966 2967Strain fitness (log2 ratio) -2 -1 0 1 2at 2964.721 kb on + strandat 2964.878 kb on - strand, within escIat 2964.878 kb on - strand, within escIat 2964.878 kb on - strand, within escIat 2964.982 kb on + strand, within escIat 2965.038 kb on + strand, within escIat 2965.160 kb on - strandat 2965.239 kb on - strand, within espZat 2965.248 kb on + strand, within espZat 2965.279 kb on + strand, within espZat 2965.322 kb on + strand, within espZat 2965.368 kb on - strand, within espZat 2965.540 kb on + strandat 2965.606 kb on + strandat 2965.612 kb on + strandat 2966.367 kb on - strand, within escVat 2966.400 kb on - strand, within escVat 2966.586 kb on + strand, within escVat 2966.685 kb on - strand, within escVat 2966.685 kb on - strand, within escVat 2966.720 kb on + strand, within escVat 2966.721 kb on - strand, within escVat 2966.855 kb on + strand, within escVat 2966.918 kb on - strand, within escV

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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2,964,721 + -2.5
2,964,878 - escI OKFHMN_15345 0.37 -0.3
2,964,878 - escI OKFHMN_15345 0.37 +0.2
2,964,878 - escI OKFHMN_15345 0.37 +0.3
2,964,982 + escI OKFHMN_15345 0.64 +0.1
2,965,038 + escI OKFHMN_15345 0.79 +0.4
2,965,160 - -0.5
2,965,239 - espZ OKFHMN_15350 0.13 -1.7
2,965,248 + espZ OKFHMN_15350 0.16 +1.1
2,965,279 + espZ OKFHMN_15350 0.26 -0.4
2,965,322 + espZ OKFHMN_15350 0.41 -0.1
2,965,368 - espZ OKFHMN_15350 0.56 -0.6
2,965,540 + +0.1
2,965,606 + +2.1
2,965,612 + +0.6
2,966,367 - escV OKFHMN_15360 0.16 -0.8
2,966,400 - escV OKFHMN_15360 0.18 -0.5
2,966,586 + escV OKFHMN_15360 0.27 +0.2
2,966,685 - escV OKFHMN_15360 0.32 -0.5
2,966,685 - escV OKFHMN_15360 0.32 +0.5
2,966,720 + escV OKFHMN_15360 0.34 -0.8
2,966,721 - escV OKFHMN_15360 0.34 +0.3
2,966,855 + escV OKFHMN_15360 0.40 -0.3
2,966,918 - escV OKFHMN_15360 0.44 -1.2

Or see this region's nucleotide sequence