Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15315

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescU and etgA overlap by 4 nucleotidesetgA and grlR are separated by 195 nucleotidesgrlR and grlA are separated by 54 nucleotidesgrlA and cesD are separated by 383 nucleotides OKFHMN_15305: escU - type III secretion system LEE export apparatus switch protein EscU, at 2,958,785 to 2,959,822 escU OKFHMN_15310: etgA - type III secretion system LEE muramidase EtgA, at 2,959,819 to 2,960,277 etgA OKFHMN_15315: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 2,960,473 to 2,960,844 grlR OKFHMN_15320: grlA - type III secretion system LEE transcriptional regulator GrlA, at 2,960,899 to 2,961,312 grlA OKFHMN_15325: cesD - SycD/LcrH family type III secretion system chaperone CesD, at 2,961,696 to 2,962,151 cesD Position (kb) 2960 2961Strain fitness (log2 ratio) -1 0 1at 2959.596 kb on - strand, within escUat 2959.599 kb on - strand, within escUat 2959.744 kb on + strandat 2959.806 kb on + strandat 2959.857 kb on - strandat 2960.193 kb on + strand, within etgAat 2960.232 kb on - strandat 2960.422 kb on - strandat 2960.539 kb on - strand, within grlRat 2960.624 kb on + strand, within grlRat 2960.690 kb on + strand, within grlRat 2960.806 kb on - strand, within grlRat 2960.974 kb on - strand, within grlAat 2961.253 kb on - strand, within grlAat 2961.271 kb on - strandat 2961.468 kb on + strandat 2961.484 kb on + strandat 2961.726 kb on - strandat 2961.761 kb on + strand, within cesDat 2961.823 kb on + strand, within cesD

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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2,959,596 - escU OKFHMN_15305 0.78 -0.1
2,959,599 - escU OKFHMN_15305 0.78 +0.2
2,959,744 + +0.6
2,959,806 + +1.3
2,959,857 - -0.5
2,960,193 + etgA OKFHMN_15310 0.81 -1.1
2,960,232 - +0.5
2,960,422 - +1.5
2,960,539 - grlR OKFHMN_15315 0.18 -0.3
2,960,624 + grlR OKFHMN_15315 0.41 +0.5
2,960,690 + grlR OKFHMN_15315 0.58 +0.1
2,960,806 - grlR OKFHMN_15315 0.90 -0.1
2,960,974 - grlA OKFHMN_15320 0.18 +0.5
2,961,253 - grlA OKFHMN_15320 0.86 +0.1
2,961,271 - +0.8
2,961,468 + +0.1
2,961,484 + +1.2
2,961,726 - +0.1
2,961,761 + cesD OKFHMN_15325 0.14 -0.2
2,961,823 + cesD OKFHMN_15325 0.28 +0.4

Or see this region's nucleotide sequence