Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dkgB and Asp_trna are separated by 162 nucleotides Asp_trna and 5S_rrna are separated by 54 nucleotides 5S_rrna and 23S_rrna are separated by 94 nucleotides 23S_rrna and Ala_trna are separated by 183 nucleotides Ala_trna and Ile_trna are separated by 42 nucleotides Ile_trna and 16S_rrna are separated by 68 nucleotides
OKFHMN_10005: dkgB - 2,5-didehydrogluconate reductase DkgB, at 1,869,893 to 1,870,696
dkgB
OKFHMN_10010: Asp_trna - tRNA-Asp(gtc), at 1,870,859 to 1,870,935
_trna
OKFHMN_10015: 5S_rrna - 5S ribosomal RNA, at 1,870,990 to 1,871,105
_rrna
OKFHMN_10020: 23S_rrna - 23S ribosomal RNA, at 1,871,200 to 1,874,102
_rrna
OKFHMN_10030: Ala_trna - tRNA-Ala(tgc), at 1,874,286 to 1,874,361
_trna
OKFHMN_10035: Ile_trna - tRNA-Ile(gat), at 1,874,404 to 1,874,480
_trna
OKFHMN_10040: 16S_rrna - 16S ribosomal RNA, at 1,874,549 to 1,876,090
_rrna
Position (kb)
1871
1872
1873
1874
1875 Strain fitness (log2 ratio)
-1
0
1 at 1870.250 kb on - strand, within dkgB at 1870.366 kb on + strand, within dkgB at 1870.429 kb on - strand, within dkgB at 1870.460 kb on + strand, within dkgB at 1870.582 kb on - strand, within dkgB at 1870.696 kb on + strand at 1870.696 kb on + strand at 1870.856 kb on - strand at 1870.856 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 1,870,250 - dkgB OKFHMN_10005 0.44 +0.0 1,870,366 + dkgB OKFHMN_10005 0.59 -1.4 1,870,429 - dkgB OKFHMN_10005 0.67 -0.7 1,870,460 + dkgB OKFHMN_10005 0.71 +0.1 1,870,582 - dkgB OKFHMN_10005 0.86 -1.1 1,870,696 + -0.7 1,870,696 + +0.3 1,870,856 - -1.2 1,870,856 - +0.1
Or see this region's nucleotide sequence