Strain Fitness in Escherichia coli ECRC100 around OKFHMN_07840

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlpxH and purE are separated by 117 nucleotidespurE and purK overlap by 4 nucleotidespurK and arcC are separated by 138 nucleotides OKFHMN_07830: lpxH - UDP-2,3-diacylglucosamine diphosphatase, at 1,426,919 to 1,427,641 lpxH OKFHMN_07835: purE - N5-carboxyaminoimidazole ribonucleotide mutase, at 1,427,759 to 1,428,268 purE OKFHMN_07840: purK - 5-(carboxyamino)imidazole ribonucleotide synthase, at 1,428,265 to 1,429,332 purK OKFHMN_07845: arcC - carbamate kinase, at 1,429,471 to 1,430,364 arcC Position (kb) 1428 1429 1430Strain fitness (log2 ratio) -3 -2 -1 0 1at 1427.705 kb on - strandat 1427.945 kb on + strand, within purEat 1428.082 kb on - strand, within purEat 1428.306 kb on + strandat 1428.602 kb on + strand, within purKat 1428.602 kb on + strand, within purKat 1428.621 kb on + strand, within purKat 1428.920 kb on - strand, within purKat 1428.920 kb on - strand, within purKat 1428.996 kb on + strand, within purKat 1429.047 kb on - strand, within purKat 1429.047 kb on - strand, within purKat 1429.125 kb on + strand, within purKat 1429.139 kb on - strand, within purKat 1429.178 kb on - strand, within purKat 1429.291 kb on + strandat 1429.292 kb on - strandat 1429.321 kb on - strandat 1429.382 kb on + strandat 1429.405 kb on - strandat 1429.684 kb on + strand, within arcCat 1430.106 kb on + strand, within arcCat 1430.162 kb on - strand, within arcCat 1430.327 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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1,427,705 - -0.6
1,427,945 + purE OKFHMN_07835 0.36 +0.7
1,428,082 - purE OKFHMN_07835 0.63 -0.9
1,428,306 + -0.7
1,428,602 + purK OKFHMN_07840 0.32 -0.1
1,428,602 + purK OKFHMN_07840 0.32 +1.2
1,428,621 + purK OKFHMN_07840 0.33 -1.3
1,428,920 - purK OKFHMN_07840 0.61 -0.3
1,428,920 - purK OKFHMN_07840 0.61 +0.6
1,428,996 + purK OKFHMN_07840 0.68 +0.1
1,429,047 - purK OKFHMN_07840 0.73 -0.9
1,429,047 - purK OKFHMN_07840 0.73 +0.1
1,429,125 + purK OKFHMN_07840 0.81 -1.5
1,429,139 - purK OKFHMN_07840 0.82 -3.6
1,429,178 - purK OKFHMN_07840 0.85 +0.7
1,429,291 + +0.3
1,429,292 - +0.1
1,429,321 - -1.1
1,429,382 + -0.2
1,429,405 - -0.2
1,429,684 + arcC OKFHMN_07845 0.24 -0.1
1,430,106 + arcC OKFHMN_07845 0.71 +0.7
1,430,162 - arcC OKFHMN_07845 0.77 +0.8
1,430,327 - -0.0

Or see this region's nucleotide sequence