Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ureC and ureB overlap by 8 nucleotides ureB and ureA are separated by 8 nucleotides ureA and ureD1 are separated by 90 nucleotides ureD1 and OKFHMN_04305 are separated by 380 nucleotides OKFHMN_04305 and opgE are separated by 224 nucleotides
OKFHMN_04280: ureC - urease subunit alpha, at 683,817 to 685,520
ureC
OKFHMN_04290: ureB - urease subunit beta, at 685,513 to 685,833
ureB
OKFHMN_04295: ureA - urease subunit gamma, at 685,842 to 686,144
ureA
OKFHMN_04300: ureD1 - Putative urease accessory UreD-like protein, at 686,235 to 686,933
ureD1
OKFHMN_04305: OKFHMN_04305 - hypothetical protein, at 687,314 to 687,589
_04305
OKFHMN_04310: opgE - phosphoethanolamine transferase, at 687,814 to 689,433
opgE
Position (kb)
686
687 Strain fitness (log2 ratio)
-1
0
1
2
3 at 685.410 kb on - strand at 685.543 kb on + strand at 685.546 kb on - strand, within ureB at 685.546 kb on - strand, within ureB at 685.774 kb on - strand, within ureB at 685.796 kb on - strand, within ureB at 685.800 kb on - strand, within ureB at 685.818 kb on - strand at 685.818 kb on - strand at 685.927 kb on - strand, within ureA at 686.018 kb on + strand, within ureA at 686.110 kb on - strand, within ureA at 686.112 kb on + strand, within ureA at 686.155 kb on + strand at 686.233 kb on - strand at 686.253 kb on - strand at 686.338 kb on - strand, within ureD1 at 687.038 kb on - strand at 687.080 kb on - strand at 687.125 kb on + strand at 687.126 kb on - strand at 687.236 kb on + strand at 687.266 kb on + strand at 687.434 kb on - strand, within OKFHMN_04305
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 685,410 - -0.5 685,543 + +1.1 685,546 - ureB OKFHMN_04290 0.10 +0.5 685,546 - ureB OKFHMN_04290 0.10 +0.5 685,774 - ureB OKFHMN_04290 0.81 +1.5 685,796 - ureB OKFHMN_04290 0.88 +0.4 685,800 - ureB OKFHMN_04290 0.89 -0.3 685,818 - -1.1 685,818 - +0.7 685,927 - ureA OKFHMN_04295 0.28 +0.4 686,018 + ureA OKFHMN_04295 0.58 -0.1 686,110 - ureA OKFHMN_04295 0.88 +0.5 686,112 + ureA OKFHMN_04295 0.89 -0.5 686,155 + +2.8 686,233 - -0.4 686,253 - +0.1 686,338 - ureD1 OKFHMN_04300 0.15 -0.1 687,038 - -0.5 687,080 - +0.8 687,125 + +0.9 687,126 - +1.1 687,236 + -0.1 687,266 + +2.1 687,434 - OKFHMN_04305 0.43 -0.2
Or see this region's nucleotide sequence