Strain Fitness in Escherichia coli ECRC100 around OKFHMN_04005

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_04000 and OKFHMN_04005 overlap by 4 nucleotidesOKFHMN_04005 and fhaC are separated by 240 nucleotides OKFHMN_04000: OKFHMN_04000 - helicase, at 638,807 to 640,975 _04000 OKFHMN_04005: OKFHMN_04005 - Putative UvrD/REP helicase-like protein, at 640,972 to 641,487 _04005 OKFHMN_04010: fhaC - ShlB/FhaC/HecB family hemolysin secretion/activation protein, at 641,728 to 642,774 fhaC Position (kb) 640 641 642Strain fitness (log2 ratio) -1 0 1at 640.070 kb on - strand, within OKFHMN_04000at 640.876 kb on + strandat 641.604 kb on - strandat 641.709 kb on + strandat 642.205 kb on - strand, within fhaCat 642.206 kb on - strand, within fhaCat 642.229 kb on - strand, within fhaCat 642.233 kb on - strand, within fhaCat 642.309 kb on + strand, within fhaC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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640,070 - OKFHMN_04000 0.58 +0.3
640,876 + -0.2
641,604 - +0.5
641,709 + +1.2
642,205 - fhaC OKFHMN_04010 0.46 +0.8
642,206 - fhaC OKFHMN_04010 0.46 -0.1
642,229 - fhaC OKFHMN_04010 0.48 -0.7
642,233 - fhaC OKFHMN_04010 0.48 -0.1
642,309 + fhaC OKFHMN_04010 0.55 +0.2

Or see this region's nucleotide sequence