Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02565

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlapB and lapA are separated by 6 nucleotideslapA and pgpB are separated by 148 nucleotidespgpB and ribA are separated by 169 nucleotidesribA and acnA are separated by 63 nucleotides OKFHMN_02555: lapB - lipopolysaccharide assembly protein LapB, at 402,481 to 403,650 lapB OKFHMN_02560: lapA - lipopolysaccharide assembly protein LapA, at 403,657 to 403,965 lapA OKFHMN_02565: pgpB - phosphatidylglycerophosphatase B, at 404,114 to 404,878 pgpB OKFHMN_02570: ribA - GTP cyclohydrolase-2, at 405,048 to 405,638 ribA OKFHMN_02575: acnA - aconitate hydratase AcnA, at 405,702 to 408,377 acnA Position (kb) 404 405Strain fitness (log2 ratio) -1 0 1at 403.865 kb on - strand, within lapAat 403.969 kb on - strandat 404.028 kb on - strandat 404.234 kb on - strand, within pgpBat 404.237 kb on + strand, within pgpBat 404.238 kb on - strand, within pgpBat 404.896 kb on - strandat 404.910 kb on + strandat 405.637 kb on - strandat 405.714 kb on - strandat 405.727 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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403,865 - lapA OKFHMN_02560 0.67 -0.6
403,969 - -1.2
404,028 - +0.2
404,234 - pgpB OKFHMN_02565 0.16 +0.5
404,237 + pgpB OKFHMN_02565 0.16 -0.2
404,238 - pgpB OKFHMN_02565 0.16 -0.0
404,896 - -0.4
404,910 + +0.8
405,637 - -0.9
405,714 - -0.7
405,727 + -0.2

Or see this region's nucleotide sequence