Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02525

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrnb and pdeR are separated by 234 nucleotidespdeR and yciZ are separated by 147 nucleotidesyciZ and yciT are separated by 89 nucleotides OKFHMN_02520: rnb - exoribonuclease II, at 395,298 to 397,232 rnb OKFHMN_02525: pdeR - cyclic di-GMP phosphodiesterase, at 397,467 to 399,452 pdeR OKFHMN_02530: yciZ - UPF0509 protein YciZ, at 399,600 to 399,773 yciZ OKFHMN_02535: yciT - DNA-binding transcriptional regulator YciT, at 399,863 to 400,612 yciT Position (kb) 397 398 399 400Strain fitness (log2 ratio) -2 -1 0 1at 396.534 kb on + strand, within rnbat 396.535 kb on - strand, within rnbat 396.771 kb on + strand, within rnbat 397.230 kb on + strandat 398.211 kb on - strand, within pdeRat 399.322 kb on + strandat 399.466 kb on + strandat 399.689 kb on + strand, within yciZat 399.700 kb on + strand, within yciZat 400.070 kb on + strand, within yciT

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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396,534 + rnb OKFHMN_02520 0.64 -0.9
396,535 - rnb OKFHMN_02520 0.64 -0.9
396,771 + rnb OKFHMN_02520 0.76 +0.4
397,230 + -0.4
398,211 - pdeR OKFHMN_02525 0.37 +0.9
399,322 + -0.2
399,466 + -0.6
399,689 + yciZ OKFHMN_02530 0.51 -1.2
399,700 + yciZ OKFHMN_02530 0.57 -1.8
400,070 + yciT OKFHMN_02535 0.28 +0.4

Or see this region's nucleotide sequence