Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02015

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMet_trna and OKFHMN_02010 are separated by 151 nucleotidesOKFHMN_02010 and OKFHMN_02015 overlap by 4 nucleotidesOKFHMN_02015 and OKFHMN_02020 overlap by 1 nucleotidesOKFHMN_02020 and OKFHMN_02025 are separated by 71 nucleotides OKFHMN_02005: Met_trna - tRNA-Met(cat), at 307,285 to 307,355 _trna OKFHMN_02010: OKFHMN_02010 - antiterminator, at 307,507 to 308,049 _02010 OKFHMN_02015: OKFHMN_02015 - DUF1364 domain-containing protein, at 308,046 to 308,336 _02015 OKFHMN_02020: OKFHMN_02020 - DUF1367 domain-containing protein, at 308,336 to 308,935 _02020 OKFHMN_02025: OKFHMN_02025 - Bacteriophage cohesive ends, at 309,007 to 309,219 _02025 Position (kb) 308 309Strain fitness (log2 ratio) -1 0 1at 307.459 kb on - strandat 307.548 kb on - strandat 307.548 kb on - strandat 307.654 kb on - strand, within OKFHMN_02010at 307.727 kb on - strand, within OKFHMN_02010at 307.912 kb on - strand, within OKFHMN_02010at 308.298 kb on - strand, within OKFHMN_02015at 308.322 kb on - strandat 308.421 kb on - strand, within OKFHMN_02020at 308.894 kb on + strandat 308.983 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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307,459 - +0.5
307,548 - -0.2
307,548 - -0.0
307,654 - OKFHMN_02010 0.27 +0.5
307,727 - OKFHMN_02010 0.41 -0.0
307,912 - OKFHMN_02010 0.75 -0.2
308,298 - OKFHMN_02015 0.87 -0.2
308,322 - +0.4
308,421 - OKFHMN_02020 0.14 +0.2
308,894 + +0.6
308,983 - -1.4

Or see this region's nucleotide sequence