Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Met_trna and OKFHMN_02010 are separated by 151 nucleotides OKFHMN_02010 and OKFHMN_02015 overlap by 4 nucleotides OKFHMN_02015 and OKFHMN_02020 overlap by 1 nucleotides OKFHMN_02020 and OKFHMN_02025 are separated by 71 nucleotides
OKFHMN_02005: Met_trna - tRNA-Met(cat), at 307,285 to 307,355
_trna
OKFHMN_02010: OKFHMN_02010 - antiterminator, at 307,507 to 308,049
_02010
OKFHMN_02015: OKFHMN_02015 - DUF1364 domain-containing protein, at 308,046 to 308,336
_02015
OKFHMN_02020: OKFHMN_02020 - DUF1367 domain-containing protein, at 308,336 to 308,935
_02020
OKFHMN_02025: OKFHMN_02025 - Bacteriophage cohesive ends, at 309,007 to 309,219
_02025
Position (kb)
308
309 Strain fitness (log2 ratio)
-1
0
1 at 307.459 kb on - strand at 307.548 kb on - strand at 307.548 kb on - strand at 307.654 kb on - strand, within OKFHMN_02010 at 307.727 kb on - strand, within OKFHMN_02010 at 307.912 kb on - strand, within OKFHMN_02010 at 308.298 kb on - strand, within OKFHMN_02015 at 308.322 kb on - strand at 308.421 kb on - strand, within OKFHMN_02020 at 308.894 kb on + strand at 308.983 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 307,459 - +0.5 307,548 - -0.2 307,548 - -0.0 307,654 - OKFHMN_02010 0.27 +0.5 307,727 - OKFHMN_02010 0.41 -0.0 307,912 - OKFHMN_02010 0.75 -0.2 308,298 - OKFHMN_02015 0.87 -0.2 308,322 - +0.4 308,421 - OKFHMN_02020 0.14 +0.2 308,894 + +0.6 308,983 - -1.4
Or see this region's nucleotide sequence