Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argB and OKGIIK_09445 are separated by 20 nucleotides OKGIIK_09445 and OKGIIK_09450 are separated by 13 nucleotides OKGIIK_09450 and OKGIIK_09455 are separated by 20 nucleotides OKGIIK_09455 and OKGIIK_09460 overlap by 4 nucleotides
OKGIIK_09440: argB - acetylglutamate kinase, at 2,048,047 to 2,049,381
argB
OKGIIK_09445: OKGIIK_09445 - acetylornithine deacetylase, at 2,049,402 to 2,050,535
_09445
OKGIIK_09450: OKGIIK_09450 - Steroid delta-isomerase, at 2,050,549 to 2,050,896
_09450
OKGIIK_09455: OKGIIK_09455 - GNAT family acetyltransferase, at 2,050,917 to 2,051,369
_09455
OKGIIK_09460: OKGIIK_09460 - DinB family, at 2,051,366 to 2,051,872
_09460
Position (kb)
2049
2050
2051 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2048.414 kb on + strand, within argB at 2048.414 kb on + strand, within argB at 2048.415 kb on - strand, within argB at 2048.415 kb on - strand, within argB at 2048.771 kb on + strand, within argB at 2048.771 kb on + strand, within argB at 2048.771 kb on + strand, within argB at 2048.771 kb on + strand, within argB at 2048.772 kb on - strand, within argB at 2048.772 kb on - strand, within argB at 2048.772 kb on - strand, within argB at 2048.957 kb on + strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2049.302 kb on + strand at 2049.302 kb on + strand at 2049.302 kb on + strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.385 kb on + strand at 2049.385 kb on + strand at 2049.385 kb on + strand at 2049.385 kb on + strand at 2049.386 kb on - strand at 2049.386 kb on - strand at 2049.386 kb on - strand at 2049.478 kb on + strand at 2049.479 kb on - strand at 2049.479 kb on - strand at 2049.548 kb on - strand, within OKGIIK_09445 at 2050.096 kb on + strand, within OKGIIK_09445 at 2050.096 kb on + strand, within OKGIIK_09445 at 2050.096 kb on + strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.430 kb on + strand at 2050.430 kb on + strand at 2050.430 kb on + strand at 2050.430 kb on + strand at 2050.431 kb on - strand at 2050.431 kb on - strand at 2050.432 kb on + strand at 2050.432 kb on + strand at 2050.432 kb on + strand at 2050.432 kb on + strand at 2050.432 kb on + strand at 2050.432 kb on + strand at 2050.433 kb on - strand at 2050.524 kb on + strand at 2050.524 kb on + strand at 2050.524 kb on + strand at 2050.524 kb on + strand at 2050.525 kb on - strand at 2050.525 kb on - strand at 2050.525 kb on - strand at 2050.525 kb on - strand at 2050.568 kb on + strand at 2050.569 kb on - strand at 2050.569 kb on - strand at 2050.569 kb on - strand at 2050.569 kb on - strand at 2050.569 kb on - strand at 2050.600 kb on + strand, within OKGIIK_09450 at 2050.600 kb on + strand, within OKGIIK_09450 at 2050.600 kb on + strand, within OKGIIK_09450 at 2050.601 kb on - strand, within OKGIIK_09450 at 2050.601 kb on - strand, within OKGIIK_09450 at 2050.601 kb on - strand, within OKGIIK_09450 at 2050.601 kb on - strand, within OKGIIK_09450 at 2050.739 kb on + strand, within OKGIIK_09450 at 2050.739 kb on + strand, within OKGIIK_09450 at 2050.739 kb on + strand, within OKGIIK_09450 at 2050.887 kb on + strand at 2050.954 kb on + strand at 2050.954 kb on + strand at 2050.954 kb on + strand at 2050.955 kb on - strand at 2050.955 kb on - strand at 2050.955 kb on - strand at 2050.955 kb on - strand at 2050.955 kb on - strand at 2050.955 kb on - strand at 2050.955 kb on - strand at 2050.955 kb on - strand at 2050.955 kb on - strand at 2050.955 kb on - strand at 2051.023 kb on + strand, within OKGIIK_09455 at 2051.023 kb on + strand, within OKGIIK_09455 at 2051.024 kb on - strand, within OKGIIK_09455 at 2051.221 kb on + strand, within OKGIIK_09455 at 2051.221 kb on + strand, within OKGIIK_09455 at 2051.221 kb on + strand, within OKGIIK_09455 at 2051.221 kb on + strand, within OKGIIK_09455 at 2051.222 kb on - strand, within OKGIIK_09455 at 2051.222 kb on - strand, within OKGIIK_09455 at 2051.222 kb on - strand, within OKGIIK_09455 at 2051.290 kb on + strand, within OKGIIK_09455 at 2051.291 kb on - strand, within OKGIIK_09455 at 2051.291 kb on - strand, within OKGIIK_09455 at 2051.291 kb on - strand, within OKGIIK_09455 at 2051.291 kb on - strand, within OKGIIK_09455 at 2051.363 kb on + strand at 2051.363 kb on + strand at 2051.363 kb on + strand at 2051.364 kb on - strand at 2051.364 kb on - strand at 2051.364 kb on - strand at 2051.364 kb on - strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.414 kb on + strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.415 kb on - strand at 2051.418 kb on + strand, within OKGIIK_09460 at 2051.418 kb on + strand, within OKGIIK_09460 at 2051.418 kb on + strand, within OKGIIK_09460 at 2051.418 kb on + strand, within OKGIIK_09460 at 2051.418 kb on + strand, within OKGIIK_09460 at 2051.419 kb on - strand, within OKGIIK_09460 at 2051.419 kb on - strand, within OKGIIK_09460 at 2051.419 kb on - strand, within OKGIIK_09460 at 2051.419 kb on - strand, within OKGIIK_09460 at 2051.419 kb on - strand, within OKGIIK_09460 at 2051.419 kb on - strand, within OKGIIK_09460 at 2051.490 kb on + strand, within OKGIIK_09460 at 2051.490 kb on + strand, within OKGIIK_09460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 2,048,414 + argB OKGIIK_09440 0.27 +2.3 2,048,414 + argB OKGIIK_09440 0.27 +0.3 2,048,415 - argB OKGIIK_09440 0.28 -0.7 2,048,415 - argB OKGIIK_09440 0.28 -0.9 2,048,771 + argB OKGIIK_09440 0.54 +0.3 2,048,771 + argB OKGIIK_09440 0.54 -0.9 2,048,771 + argB OKGIIK_09440 0.54 -0.5 2,048,771 + argB OKGIIK_09440 0.54 -0.0 2,048,772 - argB OKGIIK_09440 0.54 +0.1 2,048,772 - argB OKGIIK_09440 0.54 -0.0 2,048,772 - argB OKGIIK_09440 0.54 -1.5 2,048,957 + argB OKGIIK_09440 0.68 -0.7 2,048,958 - argB OKGIIK_09440 0.68 +0.7 2,048,958 - argB OKGIIK_09440 0.68 +0.1 2,048,958 - argB OKGIIK_09440 0.68 +1.9 2,048,958 - argB OKGIIK_09440 0.68 -1.7 2,048,958 - argB OKGIIK_09440 0.68 +0.2 2,048,958 - argB OKGIIK_09440 0.68 -2.0 2,048,958 - argB OKGIIK_09440 0.68 -0.4 2,048,958 - argB OKGIIK_09440 0.68 +1.7 2,048,958 - argB OKGIIK_09440 0.68 -0.9 2,049,302 + +0.7 2,049,302 + +1.5 2,049,302 + -0.8 2,049,303 - +1.3 2,049,303 - +0.3 2,049,303 - -1.0 2,049,303 - -0.2 2,049,303 - +0.3 2,049,303 - -0.7 2,049,303 - -0.1 2,049,303 - +0.5 2,049,385 + -0.7 2,049,385 + +0.6 2,049,385 + +0.9 2,049,385 + +0.9 2,049,386 - +0.1 2,049,386 - -0.3 2,049,386 - -0.7 2,049,478 + -0.1 2,049,479 - -1.0 2,049,479 - +1.6 2,049,548 - OKGIIK_09445 0.13 -0.3 2,050,096 + OKGIIK_09445 0.61 +0.3 2,050,096 + OKGIIK_09445 0.61 +1.1 2,050,096 + OKGIIK_09445 0.61 -0.7 2,050,097 - OKGIIK_09445 0.61 -1.2 2,050,097 - OKGIIK_09445 0.61 -1.2 2,050,097 - OKGIIK_09445 0.61 +0.1 2,050,097 - OKGIIK_09445 0.61 -0.2 2,050,097 - OKGIIK_09445 0.61 +1.8 2,050,097 - OKGIIK_09445 0.61 +0.8 2,050,097 - OKGIIK_09445 0.61 +0.5 2,050,430 + +0.4 2,050,430 + -0.7 2,050,430 + +1.3 2,050,430 + +0.5 2,050,431 - -2.4 2,050,431 - -2.0 2,050,432 + -0.1 2,050,432 + -1.9 2,050,432 + +0.1 2,050,432 + +0.4 2,050,432 + +0.3 2,050,432 + -0.7 2,050,433 - +0.9 2,050,524 + +0.8 2,050,524 + -0.5 2,050,524 + +1.3 2,050,524 + -0.1 2,050,525 - -0.3 2,050,525 - -2.0 2,050,525 - -1.9 2,050,525 - +1.3 2,050,568 + -0.7 2,050,569 - +0.8 2,050,569 - +2.0 2,050,569 - -0.1 2,050,569 - -1.5 2,050,569 - +0.2 2,050,600 + OKGIIK_09450 0.15 -0.2 2,050,600 + OKGIIK_09450 0.15 -0.1 2,050,600 + OKGIIK_09450 0.15 -0.4 2,050,601 - OKGIIK_09450 0.15 +0.1 2,050,601 - OKGIIK_09450 0.15 -0.0 2,050,601 - OKGIIK_09450 0.15 -0.1 2,050,601 - OKGIIK_09450 0.15 +1.9 2,050,739 + OKGIIK_09450 0.55 +0.1 2,050,739 + OKGIIK_09450 0.55 -0.0 2,050,739 + OKGIIK_09450 0.55 +1.1 2,050,887 + +0.6 2,050,954 + -3.0 2,050,954 + -2.0 2,050,954 + -0.5 2,050,955 - -1.9 2,050,955 - +0.9 2,050,955 - +0.2 2,050,955 - -1.7 2,050,955 - -2.3 2,050,955 - -0.5 2,050,955 - +1.3 2,050,955 - +0.3 2,050,955 - -1.4 2,050,955 - -0.3 2,051,023 + OKGIIK_09455 0.23 -0.6 2,051,023 + OKGIIK_09455 0.23 +1.5 2,051,024 - OKGIIK_09455 0.24 -0.1 2,051,221 + OKGIIK_09455 0.67 +0.3 2,051,221 + OKGIIK_09455 0.67 -1.8 2,051,221 + OKGIIK_09455 0.67 -0.4 2,051,221 + OKGIIK_09455 0.67 -0.5 2,051,222 - OKGIIK_09455 0.67 +0.9 2,051,222 - OKGIIK_09455 0.67 +0.7 2,051,222 - OKGIIK_09455 0.67 -1.0 2,051,290 + OKGIIK_09455 0.82 +1.6 2,051,291 - OKGIIK_09455 0.83 -0.0 2,051,291 - OKGIIK_09455 0.83 -0.2 2,051,291 - OKGIIK_09455 0.83 -1.3 2,051,291 - OKGIIK_09455 0.83 -0.2 2,051,363 + -0.5 2,051,363 + -0.5 2,051,363 + -2.2 2,051,364 - +0.1 2,051,364 - +0.1 2,051,364 - -0.2 2,051,364 - +0.7 2,051,414 + -0.2 2,051,414 + +0.1 2,051,414 + +0.3 2,051,414 + +1.2 2,051,414 + -0.1 2,051,414 + -0.3 2,051,414 + -0.5 2,051,414 + -1.2 2,051,414 + +0.4 2,051,414 + +0.9 2,051,414 + +0.7 2,051,414 + -1.0 2,051,414 + -1.5 2,051,414 + -1.0 2,051,415 - -2.3 2,051,415 - -0.0 2,051,415 - +0.7 2,051,415 - +0.5 2,051,415 - -1.4 2,051,415 - -0.7 2,051,415 - +0.4 2,051,415 - +0.8 2,051,415 - -1.3 2,051,415 - +0.4 2,051,415 - +0.1 2,051,415 - -0.1 2,051,415 - +1.6 2,051,415 - +0.6 2,051,415 - -0.4 2,051,418 + OKGIIK_09460 0.10 -0.7 2,051,418 + OKGIIK_09460 0.10 +0.3 2,051,418 + OKGIIK_09460 0.10 +0.1 2,051,418 + OKGIIK_09460 0.10 +0.3 2,051,418 + OKGIIK_09460 0.10 +1.3 2,051,419 - OKGIIK_09460 0.10 -0.1 2,051,419 - OKGIIK_09460 0.10 -0.5 2,051,419 - OKGIIK_09460 0.10 +0.3 2,051,419 - OKGIIK_09460 0.10 -2.2 2,051,419 - OKGIIK_09460 0.10 -0.9 2,051,419 - OKGIIK_09460 0.10 -2.5 2,051,490 + OKGIIK_09460 0.24 +1.1 2,051,490 + OKGIIK_09460 0.24 +1.1
Or see this region's nucleotide sequence