Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_02105 and OKGIIK_02110 are separated by 74 nucleotides OKGIIK_02110 and ttdA are separated by 177 nucleotides
OKGIIK_02105: OKGIIK_02105 - amino acid permease, at 488,788 to 490,113
_02105
OKGIIK_02110: OKGIIK_02110 - Phenazine biosynthesis protein PhzF, at 490,188 to 491,069
_02110
OKGIIK_02115: ttdA - fumarate hydratase, at 491,247 to 492,764
ttdA
Position (kb) 490
491
492 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 489.292 kb on + strand, within OKGIIK_02105 at 489.292 kb on + strand, within OKGIIK_02105 at 489.292 kb on + strand, within OKGIIK_02105 at 489.293 kb on - strand, within OKGIIK_02105 at 489.362 kb on + strand, within OKGIIK_02105 at 489.362 kb on + strand, within OKGIIK_02105 at 489.362 kb on + strand, within OKGIIK_02105 at 489.362 kb on + strand, within OKGIIK_02105 at 489.362 kb on + strand, within OKGIIK_02105 at 489.362 kb on + strand, within OKGIIK_02105 at 489.362 kb on + strand, within OKGIIK_02105 at 489.363 kb on - strand, within OKGIIK_02105 at 489.363 kb on - strand, within OKGIIK_02105 at 489.363 kb on - strand, within OKGIIK_02105 at 489.383 kb on + strand, within OKGIIK_02105 at 489.383 kb on + strand, within OKGIIK_02105 at 489.383 kb on + strand, within OKGIIK_02105 at 489.384 kb on - strand, within OKGIIK_02105 at 489.384 kb on - strand, within OKGIIK_02105 at 489.384 kb on - strand, within OKGIIK_02105 at 489.384 kb on - strand, within OKGIIK_02105 at 489.448 kb on + strand, within OKGIIK_02105 at 489.448 kb on + strand, within OKGIIK_02105 at 489.448 kb on + strand, within OKGIIK_02105 at 489.448 kb on + strand, within OKGIIK_02105 at 489.449 kb on - strand, within OKGIIK_02105 at 489.449 kb on - strand, within OKGIIK_02105 at 489.473 kb on + strand, within OKGIIK_02105 at 489.473 kb on + strand, within OKGIIK_02105 at 489.473 kb on + strand, within OKGIIK_02105 at 489.473 kb on + strand, within OKGIIK_02105 at 489.473 kb on + strand, within OKGIIK_02105 at 489.473 kb on + strand, within OKGIIK_02105 at 489.473 kb on + strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.474 kb on - strand, within OKGIIK_02105 at 489.488 kb on + strand, within OKGIIK_02105 at 489.488 kb on + strand, within OKGIIK_02105 at 489.489 kb on - strand, within OKGIIK_02105 at 489.800 kb on + strand, within OKGIIK_02105 at 489.800 kb on + strand, within OKGIIK_02105 at 489.800 kb on + strand, within OKGIIK_02105 at 489.800 kb on + strand, within OKGIIK_02105 at 489.800 kb on + strand, within OKGIIK_02105 at 489.800 kb on + strand, within OKGIIK_02105 at 489.800 kb on + strand, within OKGIIK_02105 at 489.800 kb on + strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.801 kb on - strand, within OKGIIK_02105 at 489.812 kb on - strand, within OKGIIK_02105 at 489.866 kb on + strand, within OKGIIK_02105 at 489.866 kb on + strand, within OKGIIK_02105 at 489.866 kb on + strand, within OKGIIK_02105 at 489.866 kb on + strand, within OKGIIK_02105 at 489.867 kb on - strand, within OKGIIK_02105 at 489.867 kb on - strand, within OKGIIK_02105 at 489.867 kb on - strand, within OKGIIK_02105 at 489.867 kb on - strand, within OKGIIK_02105 at 489.867 kb on - strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.872 kb on + strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.873 kb on - strand, within OKGIIK_02105 at 489.914 kb on + strand, within OKGIIK_02105 at 489.914 kb on + strand, within OKGIIK_02105 at 489.915 kb on - strand, within OKGIIK_02105 at 490.085 kb on + strand at 490.085 kb on + strand at 490.085 kb on + strand at 490.085 kb on + strand at 490.085 kb on + strand at 490.085 kb on + strand at 490.085 kb on + strand at 490.085 kb on + strand at 490.085 kb on + strand at 490.085 kb on + strand at 490.085 kb on + strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.086 kb on - strand at 490.885 kb on + strand, within OKGIIK_02110 at 490.886 kb on - strand, within OKGIIK_02110 at 491.185 kb on + strand at 491.185 kb on + strand at 491.185 kb on + strand at 491.185 kb on + strand at 491.185 kb on + strand at 491.185 kb on + strand at 491.185 kb on + strand at 491.185 kb on + strand at 491.186 kb on - strand at 491.186 kb on - strand at 491.186 kb on - strand at 491.186 kb on - strand at 491.186 kb on - strand at 491.301 kb on + strand at 491.302 kb on - strand at 491.302 kb on - strand at 491.310 kb on + strand at 491.313 kb on + strand at 491.328 kb on + strand at 491.352 kb on + strand at 491.553 kb on + strand, within ttdA at 491.553 kb on + strand, within ttdA at 491.554 kb on - strand, within ttdA at 491.554 kb on - strand, within ttdA at 491.949 kb on + strand, within ttdA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 489,292 + OKGIIK_02105 0.38 -1.2 489,292 + OKGIIK_02105 0.38 -0.8 489,292 + OKGIIK_02105 0.38 -0.7 489,293 - OKGIIK_02105 0.38 -0.7 489,362 + OKGIIK_02105 0.43 +0.7 489,362 + OKGIIK_02105 0.43 -0.7 489,362 + OKGIIK_02105 0.43 -0.5 489,362 + OKGIIK_02105 0.43 +0.3 489,362 + OKGIIK_02105 0.43 -2.0 489,362 + OKGIIK_02105 0.43 +1.3 489,362 + OKGIIK_02105 0.43 -1.5 489,363 - OKGIIK_02105 0.43 -1.0 489,363 - OKGIIK_02105 0.43 +0.3 489,363 - OKGIIK_02105 0.43 -0.8 489,383 + OKGIIK_02105 0.45 -0.7 489,383 + OKGIIK_02105 0.45 +0.3 489,383 + OKGIIK_02105 0.45 +0.3 489,384 - OKGIIK_02105 0.45 +0.6 489,384 - OKGIIK_02105 0.45 -1.1 489,384 - OKGIIK_02105 0.45 -1.0 489,384 - OKGIIK_02105 0.45 +0.9 489,448 + OKGIIK_02105 0.50 -1.1 489,448 + OKGIIK_02105 0.50 -0.8 489,448 + OKGIIK_02105 0.50 +0.2 489,448 + OKGIIK_02105 0.50 -1.0 489,449 - OKGIIK_02105 0.50 -0.3 489,449 - OKGIIK_02105 0.50 -0.4 489,473 + OKGIIK_02105 0.52 -2.2 489,473 + OKGIIK_02105 0.52 +0.1 489,473 + OKGIIK_02105 0.52 +0.3 489,473 + OKGIIK_02105 0.52 -0.7 489,473 + OKGIIK_02105 0.52 +2.8 489,473 + OKGIIK_02105 0.52 -1.5 489,473 + OKGIIK_02105 0.52 -1.1 489,474 - OKGIIK_02105 0.52 -0.7 489,474 - OKGIIK_02105 0.52 -0.3 489,474 - OKGIIK_02105 0.52 +0.2 489,474 - OKGIIK_02105 0.52 -1.0 489,474 - OKGIIK_02105 0.52 +1.7 489,474 - OKGIIK_02105 0.52 +1.1 489,474 - OKGIIK_02105 0.52 +0.3 489,474 - OKGIIK_02105 0.52 +0.5 489,474 - OKGIIK_02105 0.52 +0.7 489,474 - OKGIIK_02105 0.52 +1.2 489,474 - OKGIIK_02105 0.52 +0.9 489,474 - OKGIIK_02105 0.52 -1.0 489,474 - OKGIIK_02105 0.52 +0.9 489,474 - OKGIIK_02105 0.52 -0.7 489,488 + OKGIIK_02105 0.53 -1.1 489,488 + OKGIIK_02105 0.53 -1.9 489,489 - OKGIIK_02105 0.53 -0.1 489,800 + OKGIIK_02105 0.76 +0.9 489,800 + OKGIIK_02105 0.76 -0.7 489,800 + OKGIIK_02105 0.76 +0.3 489,800 + OKGIIK_02105 0.76 +1.7 489,800 + OKGIIK_02105 0.76 -1.3 489,800 + OKGIIK_02105 0.76 +1.9 489,800 + OKGIIK_02105 0.76 -1.0 489,800 + OKGIIK_02105 0.76 -0.3 489,801 - OKGIIK_02105 0.76 -0.1 489,801 - OKGIIK_02105 0.76 -0.1 489,801 - OKGIIK_02105 0.76 +1.9 489,801 - OKGIIK_02105 0.76 -0.7 489,801 - OKGIIK_02105 0.76 +1.8 489,801 - OKGIIK_02105 0.76 -1.1 489,801 - OKGIIK_02105 0.76 +1.2 489,801 - OKGIIK_02105 0.76 -1.8 489,801 - OKGIIK_02105 0.76 -2.7 489,801 - OKGIIK_02105 0.76 +0.9 489,801 - OKGIIK_02105 0.76 -1.0 489,801 - OKGIIK_02105 0.76 +0.4 489,812 - OKGIIK_02105 0.77 +2.1 489,866 + OKGIIK_02105 0.81 -0.4 489,866 + OKGIIK_02105 0.81 -0.0 489,866 + OKGIIK_02105 0.81 -1.0 489,866 + OKGIIK_02105 0.81 +1.0 489,867 - OKGIIK_02105 0.81 -0.8 489,867 - OKGIIK_02105 0.81 +1.1 489,867 - OKGIIK_02105 0.81 -1.0 489,867 - OKGIIK_02105 0.81 +0.9 489,867 - OKGIIK_02105 0.81 +0.7 489,872 + OKGIIK_02105 0.82 +1.2 489,872 + OKGIIK_02105 0.82 +0.2 489,872 + OKGIIK_02105 0.82 +1.1 489,872 + OKGIIK_02105 0.82 +0.8 489,872 + OKGIIK_02105 0.82 -1.0 489,872 + OKGIIK_02105 0.82 -0.3 489,872 + OKGIIK_02105 0.82 -1.7 489,872 + OKGIIK_02105 0.82 -1.3 489,872 + OKGIIK_02105 0.82 +0.9 489,872 + OKGIIK_02105 0.82 -0.6 489,872 + OKGIIK_02105 0.82 -0.5 489,872 + OKGIIK_02105 0.82 -0.8 489,872 + OKGIIK_02105 0.82 -0.7 489,872 + OKGIIK_02105 0.82 +0.0 489,872 + OKGIIK_02105 0.82 -1.0 489,872 + OKGIIK_02105 0.82 +1.1 489,872 + OKGIIK_02105 0.82 -1.3 489,872 + OKGIIK_02105 0.82 -1.7 489,872 + OKGIIK_02105 0.82 -2.9 489,872 + OKGIIK_02105 0.82 -1.2 489,872 + OKGIIK_02105 0.82 +0.3 489,872 + OKGIIK_02105 0.82 +1.5 489,872 + OKGIIK_02105 0.82 -1.4 489,872 + OKGIIK_02105 0.82 -0.0 489,872 + OKGIIK_02105 0.82 +1.1 489,872 + OKGIIK_02105 0.82 -1.0 489,872 + OKGIIK_02105 0.82 -2.5 489,872 + OKGIIK_02105 0.82 -1.3 489,873 - OKGIIK_02105 0.82 +1.3 489,873 - OKGIIK_02105 0.82 -0.5 489,873 - OKGIIK_02105 0.82 +1.1 489,873 - OKGIIK_02105 0.82 +0.6 489,873 - OKGIIK_02105 0.82 -0.8 489,873 - OKGIIK_02105 0.82 -2.4 489,873 - OKGIIK_02105 0.82 -1.3 489,873 - OKGIIK_02105 0.82 -0.7 489,873 - OKGIIK_02105 0.82 -0.3 489,873 - OKGIIK_02105 0.82 +0.9 489,873 - OKGIIK_02105 0.82 +0.3 489,873 - OKGIIK_02105 0.82 -0.8 489,873 - OKGIIK_02105 0.82 -0.2 489,873 - OKGIIK_02105 0.82 -1.5 489,873 - OKGIIK_02105 0.82 +2.0 489,873 - OKGIIK_02105 0.82 -0.1 489,873 - OKGIIK_02105 0.82 -0.5 489,873 - OKGIIK_02105 0.82 -2.2 489,873 - OKGIIK_02105 0.82 -0.0 489,873 - OKGIIK_02105 0.82 +0.5 489,873 - OKGIIK_02105 0.82 +1.5 489,873 - OKGIIK_02105 0.82 +0.8 489,873 - OKGIIK_02105 0.82 +0.1 489,914 + OKGIIK_02105 0.85 -1.7 489,914 + OKGIIK_02105 0.85 -1.5 489,915 - OKGIIK_02105 0.85 +0.1 490,085 + +1.3 490,085 + +0.6 490,085 + +0.5 490,085 + -2.3 490,085 + -0.2 490,085 + +0.3 490,085 + -1.3 490,085 + -1.0 490,085 + -0.8 490,085 + -1.3 490,085 + +0.3 490,086 - -2.0 490,086 - +1.9 490,086 - +0.4 490,086 - +0.3 490,086 - -0.4 490,086 - -0.4 490,086 - +0.3 490,086 - -0.5 490,086 - +0.2 490,086 - +0.7 490,086 - -1.8 490,086 - +1.1 490,086 - -0.9 490,086 - -1.0 490,086 - +0.2 490,086 - -3.1 490,086 - -1.0 490,086 - +1.3 490,086 - +0.1 490,086 - -0.7 490,885 + OKGIIK_02110 0.79 +0.2 490,886 - OKGIIK_02110 0.79 -1.0 491,185 + -0.5 491,185 + +0.1 491,185 + -1.2 491,185 + -0.7 491,185 + +1.3 491,185 + -0.1 491,185 + -0.8 491,185 + -1.1 491,186 - -0.6 491,186 - -0.9 491,186 - +0.9 491,186 - +0.2 491,186 - +0.8 491,301 + -0.5 491,302 - +1.2 491,302 - +0.3 491,310 + -0.3 491,313 + -1.0 491,328 + -0.7 491,352 + -2.0 491,553 + ttdA OKGIIK_02115 0.20 +0.3 491,553 + ttdA OKGIIK_02115 0.20 +0.6 491,554 - ttdA OKGIIK_02115 0.20 +0.3 491,554 - ttdA OKGIIK_02115 0.20 +0.6 491,949 + ttdA OKGIIK_02115 0.46 +0.6
Or see this region's nucleotide sequence