Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28650

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntoppD and oppF overlap by 4 nucleotidesoppF and cls are separated by 52 nucleotidescls and clsA are separated by 34 nucleotides OKFHMN_28645: oppD - murein tripeptide/oligopeptide ABC transporter ATP-binding protein OppD, at 5,496,215 to 5,497,228 oppD OKFHMN_28650: oppF - murein tripeptide/oligopeptide ABC transporter ATP-binding protein OppF, at 5,497,225 to 5,498,229 oppF OKFHMN_28655: cls - cardiolipin synthase, at 5,498,282 to 5,498,611 cls OKFHMN_28660: clsA - cardiolipin synthase ClsA, at 5,498,646 to 5,500,106 clsA Position (kb) 5497 5498 5499Strain fitness (log2 ratio) -1 0 1at 5496.351 kb on + strand, within oppDat 5496.572 kb on + strand, within oppDat 5496.572 kb on + strand, within oppDat 5496.765 kb on + strand, within oppDat 5497.410 kb on + strand, within oppFat 5498.280 kb on - strandat 5498.627 kb on - strandat 5499.189 kb on - strand, within clsA

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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5,496,351 + oppD OKFHMN_28645 0.13 -0.1
5,496,572 + oppD OKFHMN_28645 0.35 +1.1
5,496,572 + oppD OKFHMN_28645 0.35 +0.7
5,496,765 + oppD OKFHMN_28645 0.54 -0.5
5,497,410 + oppF OKFHMN_28650 0.18 +0.4
5,498,280 - +0.5
5,498,627 - +1.1
5,499,189 - clsA OKFHMN_28660 0.37 -0.7

Or see this region's nucleotide sequence