Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28555

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnarH and narJ overlap by 4 nucleotidesnarJ and narI overlap by 1 nucleotidesnarI and Tyr_trna are separated by 539 nucleotidesTyr_trna and Tyr_trna are separated by 209 nucleotides OKFHMN_28545: narH - nitrate reductase subunit beta, at 5,477,444 to 5,478,982 narH OKFHMN_28550: narJ - nitrate reductase molybdenum cofactor assembly chaperone, at 5,478,979 to 5,479,689 narJ OKFHMN_28555: narI - respiratory nitrate reductase subunit gamma, at 5,479,689 to 5,480,366 narI OKFHMN_28560: Tyr_trna - tRNA-Tyr(gta), at 5,480,906 to 5,480,990 _trna OKFHMN_28565: Tyr_trna - tRNA-Tyr(gta), at 5,481,200 to 5,481,284 _trna Position (kb) 5479 5480 5481Strain fitness (log2 ratio) -2 -1 0 1at 5478.798 kb on - strand, within narHat 5478.798 kb on - strand, within narHat 5478.998 kb on + strandat 5479.657 kb on + strandat 5479.723 kb on - strandat 5479.728 kb on + strandat 5479.758 kb on - strand, within narIat 5480.138 kb on + strand, within narIat 5480.336 kb on + strandat 5480.375 kb on + strandat 5481.014 kb on - strandat 5481.044 kb on - strandat 5481.120 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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5,478,798 - narH OKFHMN_28545 0.88 -0.6
5,478,798 - narH OKFHMN_28545 0.88 -0.5
5,478,998 + +0.4
5,479,657 + +1.0
5,479,723 - -0.8
5,479,728 + -0.9
5,479,758 - narI OKFHMN_28555 0.10 +0.7
5,480,138 + narI OKFHMN_28555 0.66 -1.9
5,480,336 + +1.4
5,480,375 + -0.3
5,481,014 - -0.3
5,481,044 - -0.2
5,481,120 - +0.6

Or see this region's nucleotide sequence