Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27610

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuidR and lacZ are separated by 390 nucleotideslacZ and uidA overlap by 7 nucleotides OKFHMN_27605: uidR - DNA-binding transcriptional regulator UidR, at 5,313,747 to 5,314,337 uidR OKFHMN_27610: lacZ - Beta-galactosidase/beta-glucuronidase, at 5,314,728 to 5,315,441 lacZ OKFHMN_27615: uidA - beta-glucuronidase, at 5,315,435 to 5,316,541 uidA Position (kb) 5314 5315 5316Strain fitness (log2 ratio) -2 -1 0 1 2at 5314.236 kb on + strand, within uidRat 5314.459 kb on + strandat 5314.733 kb on - strandat 5314.914 kb on + strand, within lacZat 5315.318 kb on + strand, within lacZat 5316.278 kb on + strand, within uidAat 5316.408 kb on + strand, within uidA

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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5,314,236 + uidR OKFHMN_27605 0.83 +0.2
5,314,459 + +2.7
5,314,733 - +0.4
5,314,914 + lacZ OKFHMN_27610 0.26 -0.7
5,315,318 + lacZ OKFHMN_27610 0.83 -0.3
5,316,278 + uidA OKFHMN_27615 0.76 -1.9
5,316,408 + uidA OKFHMN_27615 0.88 +2.1

Or see this region's nucleotide sequence