Experiment: EV146
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheT and OKFHMN_27090 are separated by 4 nucleotides OKFHMN_27090 and btuC are separated by 100 nucleotides btuC and btuE are separated by 62 nucleotides btuE and btuD overlap by 1 nucleotides
OKFHMN_27085: pheT - phenylalanine--tRNA ligase subunit beta, at 5,212,762 to 5,215,149
pheT
OKFHMN_27090: OKFHMN_27090 - Integration host factor subunit alpha, at 5,215,154 to 5,215,453
_27090
OKFHMN_27100: btuC - vitamin B12 ABC transporter permease BtuC, at 5,215,554 to 5,216,534
btuC
OKFHMN_27105: btuE - bifunctional thioredoxin/glutathione peroxidase, at 5,216,597 to 5,217,148
btuE
OKFHMN_27110: btuD - vitamin B12 ABC transporter ATP-binding protein BtuD, at 5,217,148 to 5,217,897
btuD
Position (kb) 5215
5216
5217 Strain fitness (log2 ratio) -1
0
1 at 5215.342 kb on + strand, within OKFHMN_27090 at 5215.465 kb on + strand at 5215.943 kb on - strand, within btuC at 5216.082 kb on + strand, within btuC at 5216.189 kb on + strand, within btuC at 5216.318 kb on + strand, within btuC at 5216.318 kb on + strand, within btuC at 5216.339 kb on + strand, within btuC at 5216.340 kb on - strand, within btuC at 5216.377 kb on + strand, within btuC at 5216.466 kb on - strand at 5216.518 kb on + strand at 5216.845 kb on + strand, within btuE at 5216.975 kb on + strand, within btuE at 5217.022 kb on + strand, within btuE at 5217.153 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EV146 remove 5,215,342 + OKFHMN_27090 0.63 +0.3 5,215,465 + -1.4 5,215,943 - btuC OKFHMN_27100 0.40 -1.2 5,216,082 + btuC OKFHMN_27100 0.54 +0.1 5,216,189 + btuC OKFHMN_27100 0.65 -0.1 5,216,318 + btuC OKFHMN_27100 0.78 -1.1 5,216,318 + btuC OKFHMN_27100 0.78 -0.5 5,216,339 + btuC OKFHMN_27100 0.80 +0.9 5,216,340 - btuC OKFHMN_27100 0.80 -0.9 5,216,377 + btuC OKFHMN_27100 0.84 +0.1 5,216,466 - -0.4 5,216,518 + +0.1 5,216,845 + btuE OKFHMN_27105 0.45 +0.6 5,216,975 + btuE OKFHMN_27105 0.68 -1.5 5,217,022 + btuE OKFHMN_27105 0.77 -0.4 5,217,153 + +0.6
Or see this region's nucleotide sequence