Experiment: EV146
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cspE and rlmA are separated by 165 nucleotides rlmA and mntP overlap by 4 nucleotides mntP and OKFHMN_26510 overlap by 4 nucleotides OKFHMN_26510 and OKFHMN_26515 are separated by 310 nucleotides OKFHMN_26515 and manZ are separated by 54 nucleotides
OKFHMN_26495: cspE - transcription antiterminator/RNA stability regulator CspE, at 5,101,154 to 5,101,363
cspE
OKFHMN_26500: rlmA - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 5,101,529 to 5,102,338
rlmA
OKFHMN_26505: mntP - manganese efflux pump MntP, at 5,102,335 to 5,102,901
mntP
OKFHMN_26510: OKFHMN_26510 - Membrane protein, at 5,102,898 to 5,103,020
_26510
OKFHMN_26515: OKFHMN_26515 - UPF0266 membrane protein YobD, at 5,103,331 to 5,103,789
_26515
OKFHMN_26520: manZ - PTS mannose transporter subunit IID, at 5,103,844 to 5,104,695
manZ
Position (kb) 5102
5103 Strain fitness (log2 ratio) -1
0
1 at 5101.390 kb on + strand at 5101.727 kb on + strand, within rlmA at 5102.112 kb on + strand, within rlmA at 5102.112 kb on + strand, within rlmA at 5102.464 kb on - strand, within mntP at 5102.866 kb on - strand at 5102.928 kb on - strand, within OKFHMN_26510 at 5102.928 kb on - strand, within OKFHMN_26510 at 5102.928 kb on - strand, within OKFHMN_26510 at 5103.021 kb on - strand at 5103.054 kb on - strand at 5103.115 kb on - strand at 5103.539 kb on - strand, within OKFHMN_26515 at 5103.835 kb on - strand at 5103.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EV146 remove 5,101,390 + -0.0 5,101,727 + rlmA OKFHMN_26500 0.24 +0.9 5,102,112 + rlmA OKFHMN_26500 0.72 -0.7 5,102,112 + rlmA OKFHMN_26500 0.72 -0.4 5,102,464 - mntP OKFHMN_26505 0.23 -0.6 5,102,866 - -0.7 5,102,928 - OKFHMN_26510 0.24 -0.4 5,102,928 - OKFHMN_26510 0.24 +0.0 5,102,928 - OKFHMN_26510 0.24 +0.5 5,103,021 - +1.6 5,103,054 - +0.4 5,103,115 - +0.6 5,103,539 - OKFHMN_26515 0.45 +0.9 5,103,835 - +0.5 5,103,846 - -1.4
Or see this region's nucleotide sequence