Strain Fitness in Escherichia coli ECRC100 around OKFHMN_21965

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdapE and yffB are separated by 3 nucleotidesyffB and ypfM are separated by 380 nucleotidesypfM and acrD are separated by 98 nucleotides OKFHMN_21955: dapE - succinyl-diaminopimelate desuccinylase, at 4,265,721 to 4,266,848 dapE OKFHMN_21960: yffB - ArsC family reductase, at 4,266,852 to 4,267,208 yffB OKFHMN_21965: ypfM - protein YpfM, at 4,267,589 to 4,267,648 ypfM OKFHMN_21970: acrD - multidrug efflux RND transporter permease AcrD, at 4,267,747 to 4,270,860 acrD Position (kb) 4267 4268Strain fitness (log2 ratio) -1 0 1 2at 4266.906 kb on - strand, within yffBat 4266.906 kb on - strand, within yffBat 4266.996 kb on - strand, within yffBat 4267.020 kb on - strand, within yffBat 4267.031 kb on + strand, within yffBat 4267.087 kb on - strand, within yffBat 4267.196 kb on - strandat 4267.196 kb on - strandat 4267.289 kb on + strandat 4267.290 kb on - strandat 4267.305 kb on + strandat 4267.315 kb on - strandat 4267.321 kb on + strandat 4267.324 kb on + strandat 4267.407 kb on + strandat 4267.420 kb on - strandat 4267.426 kb on - strandat 4267.452 kb on + strandat 4267.453 kb on - strandat 4267.476 kb on - strandat 4267.478 kb on + strandat 4267.478 kb on + strandat 4267.478 kb on + strandat 4267.478 kb on + strandat 4267.479 kb on - strandat 4267.482 kb on + strandat 4267.483 kb on - strandat 4267.507 kb on - strandat 4267.527 kb on - strandat 4267.531 kb on + strandat 4267.546 kb on - strandat 4267.546 kb on - strandat 4267.547 kb on + strandat 4267.547 kb on + strandat 4267.548 kb on - strandat 4267.548 kb on - strandat 4267.548 kb on - strandat 4267.563 kb on + strandat 4267.564 kb on - strandat 4267.569 kb on + strandat 4267.570 kb on - strandat 4267.573 kb on + strandat 4267.574 kb on - strandat 4267.574 kb on - strandat 4267.748 kb on + strandat 4267.749 kb on - strandat 4267.811 kb on + strandat 4267.824 kb on + strandat 4267.825 kb on - strandat 4268.193 kb on - strand, within acrDat 4268.216 kb on - strand, within acrDat 4268.340 kb on + strand, within acrDat 4268.412 kb on - strand, within acrDat 4268.487 kb on + strand, within acrDat 4268.553 kb on - strand, within acrDat 4268.594 kb on + strand, within acrDat 4268.594 kb on + strand, within acrDat 4268.594 kb on + strand, within acrDat 4268.628 kb on + strand, within acrD

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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4,266,906 - yffB OKFHMN_21960 0.15 -0.4
4,266,906 - yffB OKFHMN_21960 0.15 +0.0
4,266,996 - yffB OKFHMN_21960 0.40 +1.0
4,267,020 - yffB OKFHMN_21960 0.47 -0.4
4,267,031 + yffB OKFHMN_21960 0.50 -0.1
4,267,087 - yffB OKFHMN_21960 0.66 +1.1
4,267,196 - +0.0
4,267,196 - +0.2
4,267,289 + +0.8
4,267,290 - -0.6
4,267,305 + -0.0
4,267,315 - -0.6
4,267,321 + +0.1
4,267,324 + +0.5
4,267,407 + +0.8
4,267,420 - -1.0
4,267,426 - +0.6
4,267,452 + +0.0
4,267,453 - +0.3
4,267,476 - -0.0
4,267,478 + +0.3
4,267,478 + -0.5
4,267,478 + +0.6
4,267,478 + -0.5
4,267,479 - -0.0
4,267,482 + +0.0
4,267,483 - +0.5
4,267,507 - +1.4
4,267,527 - -0.6
4,267,531 + +0.7
4,267,546 - +0.4
4,267,546 - +1.1
4,267,547 + +0.5
4,267,547 + -0.0
4,267,548 - +0.5
4,267,548 - +1.0
4,267,548 - -0.6
4,267,563 + +1.3
4,267,564 - +0.7
4,267,569 + +0.3
4,267,570 - -1.2
4,267,573 + +0.2
4,267,574 - +0.1
4,267,574 - +1.2
4,267,748 + +0.2
4,267,749 - +0.8
4,267,811 + +0.2
4,267,824 + -0.0
4,267,825 - +1.8
4,268,193 - acrD OKFHMN_21970 0.14 +0.3
4,268,216 - acrD OKFHMN_21970 0.15 -1.0
4,268,340 + acrD OKFHMN_21970 0.19 +1.0
4,268,412 - acrD OKFHMN_21970 0.21 -1.0
4,268,487 + acrD OKFHMN_21970 0.24 +1.0
4,268,553 - acrD OKFHMN_21970 0.26 -0.5
4,268,594 + acrD OKFHMN_21970 0.27 -0.6
4,268,594 + acrD OKFHMN_21970 0.27 -0.0
4,268,594 + acrD OKFHMN_21970 0.27 +0.2
4,268,628 + acrD OKFHMN_21970 0.28 +0.4

Or see this region's nucleotide sequence