Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20990

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_20985 and OKFHMN_20990 are separated by 75 nucleotidesOKFHMN_20990 and tra8 are separated by 35 nucleotidestra8 and OKFHMN_21000 are separated by 190 nucleotides OKFHMN_20985: OKFHMN_20985 - Procyclic acidic repetitive protein (PARP), at 4,081,712 to 4,082,914 _20985 OKFHMN_20990: OKFHMN_20990 - SmpA-OmlA domain-containing protein, at 4,082,990 to 4,083,292 _20990 OKFHMN_20995: tra8 - IS30 family transposase, at 4,083,328 to 4,084,083 tra8 OKFHMN_21000: OKFHMN_21000 - hypothetical protein, at 4,084,274 to 4,084,390 _21000 Position (kb) 4082 4083 4084Strain fitness (log2 ratio) -2 -1 0 1at 4082.561 kb on + strand, within OKFHMN_20985at 4082.664 kb on + strand, within OKFHMN_20985at 4082.892 kb on + strandat 4082.928 kb on - strandat 4082.942 kb on + strandat 4082.943 kb on - strandat 4083.036 kb on + strand, within OKFHMN_20990at 4083.036 kb on + strand, within OKFHMN_20990at 4083.037 kb on - strand, within OKFHMN_20990at 4083.154 kb on - strand, within OKFHMN_20990at 4083.154 kb on - strand, within OKFHMN_20990at 4083.157 kb on + strand, within OKFHMN_20990at 4083.157 kb on + strand, within OKFHMN_20990at 4083.157 kb on + strand, within OKFHMN_20990at 4083.158 kb on - strand, within OKFHMN_20990at 4083.162 kb on + strand, within OKFHMN_20990at 4083.163 kb on - strand, within OKFHMN_20990at 4083.163 kb on - strand, within OKFHMN_20990at 4083.222 kb on - strand, within OKFHMN_20990at 4083.344 kb on - strandat 4083.346 kb on - strandat 4084.185 kb on - strandat 4084.282 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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4,082,561 + OKFHMN_20985 0.71 -0.8
4,082,664 + OKFHMN_20985 0.79 +0.1
4,082,892 + -0.2
4,082,928 - -1.1
4,082,942 + +0.5
4,082,943 - -0.1
4,083,036 + OKFHMN_20990 0.15 -0.1
4,083,036 + OKFHMN_20990 0.15 +0.2
4,083,037 - OKFHMN_20990 0.16 -0.1
4,083,154 - OKFHMN_20990 0.54 +0.3
4,083,154 - OKFHMN_20990 0.54 -2.0
4,083,157 + OKFHMN_20990 0.55 -0.9
4,083,157 + OKFHMN_20990 0.55 +0.2
4,083,157 + OKFHMN_20990 0.55 -1.4
4,083,158 - OKFHMN_20990 0.55 -0.5
4,083,162 + OKFHMN_20990 0.57 +0.8
4,083,163 - OKFHMN_20990 0.57 +0.0
4,083,163 - OKFHMN_20990 0.57 -0.6
4,083,222 - OKFHMN_20990 0.77 +0.4
4,083,344 - +0.4
4,083,346 - -0.4
4,084,185 - +0.2
4,084,282 - -0.2

Or see this region's nucleotide sequence