Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20555

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthypD and hypC overlap by 1 nucleotideshypC and hypB overlap by 10 nucleotideshypB and hypA are separated by 3 nucleotides OKFHMN_20550: hypD - hydrogenase formation protein HypD, at 3,999,449 to 4,000,570 hypD OKFHMN_20555: hypC - hydrogenase 3 maturation protein HypC, at 4,000,570 to 4,000,842 hypC OKFHMN_20560: hypB - hydrogenase nickel incorporation protein HypB, at 4,000,833 to 4,001,705 hypB OKFHMN_20565: hypA - hydrogenase maturation nickel metallochaperone HypA, at 4,001,709 to 4,002,059 hypA Position (kb) 4000 4001Strain fitness (log2 ratio) -2 -1 0 1at 3999.821 kb on + strand, within hypDat 3999.962 kb on - strand, within hypDat 3999.964 kb on + strand, within hypDat 3999.964 kb on + strand, within hypDat 3999.965 kb on - strand, within hypDat 4000.042 kb on + strand, within hypDat 4000.042 kb on + strand, within hypDat 4000.043 kb on - strand, within hypDat 4000.045 kb on + strand, within hypDat 4000.045 kb on + strand, within hypDat 4000.046 kb on - strand, within hypDat 4000.046 kb on - strand, within hypDat 4000.046 kb on - strand, within hypDat 4000.046 kb on - strand, within hypDat 4000.289 kb on - strand, within hypDat 4000.439 kb on - strand, within hypDat 4000.586 kb on + strandat 4000.666 kb on - strand, within hypCat 4000.670 kb on - strand, within hypCat 4000.693 kb on + strand, within hypCat 4000.694 kb on - strand, within hypCat 4000.700 kb on - strand, within hypCat 4000.754 kb on - strand, within hypCat 4001.147 kb on + strand, within hypBat 4001.331 kb on + strand, within hypBat 4001.441 kb on + strand, within hypBat 4001.527 kb on + strand, within hypBat 4001.668 kb on + strandat 4001.668 kb on + strandat 4001.699 kb on + strandat 4001.824 kb on + strand, within hypA

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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3,999,821 + hypD OKFHMN_20550 0.33 +0.3
3,999,962 - hypD OKFHMN_20550 0.46 +0.7
3,999,964 + hypD OKFHMN_20550 0.46 -0.8
3,999,964 + hypD OKFHMN_20550 0.46 +1.3
3,999,965 - hypD OKFHMN_20550 0.46 +1.1
4,000,042 + hypD OKFHMN_20550 0.53 -0.2
4,000,042 + hypD OKFHMN_20550 0.53 +0.5
4,000,043 - hypD OKFHMN_20550 0.53 -0.0
4,000,045 + hypD OKFHMN_20550 0.53 +0.6
4,000,045 + hypD OKFHMN_20550 0.53 -0.4
4,000,046 - hypD OKFHMN_20550 0.53 -0.3
4,000,046 - hypD OKFHMN_20550 0.53 +0.2
4,000,046 - hypD OKFHMN_20550 0.53 -0.0
4,000,046 - hypD OKFHMN_20550 0.53 -0.2
4,000,289 - hypD OKFHMN_20550 0.75 -0.2
4,000,439 - hypD OKFHMN_20550 0.88 -1.5
4,000,586 + +0.6
4,000,666 - hypC OKFHMN_20555 0.35 +0.7
4,000,670 - hypC OKFHMN_20555 0.37 -0.0
4,000,693 + hypC OKFHMN_20555 0.45 -0.3
4,000,694 - hypC OKFHMN_20555 0.45 -0.9
4,000,700 - hypC OKFHMN_20555 0.48 +1.2
4,000,754 - hypC OKFHMN_20555 0.67 +0.4
4,001,147 + hypB OKFHMN_20560 0.36 +0.7
4,001,331 + hypB OKFHMN_20560 0.57 +0.7
4,001,441 + hypB OKFHMN_20560 0.70 +0.2
4,001,527 + hypB OKFHMN_20560 0.79 -1.6
4,001,668 + -0.8
4,001,668 + -0.7
4,001,699 + -1.9
4,001,824 + hypA OKFHMN_20565 0.33 +0.5

Or see this region's nucleotide sequence