Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20455

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysN and cysC overlap by 1 nucleotidescysC and OKFHMN_20455 are separated by 49 nucleotidesOKFHMN_20455 and ftsB are separated by 193 nucleotidesftsB and ispD are separated by 18 nucleotides OKFHMN_20445: cysN - sulfate adenylyltransferase subunit CysN, at 3,980,447 to 3,981,874 cysN OKFHMN_20450: cysC - Adenylyl-sulfate kinase, at 3,981,874 to 3,982,479 cysC OKFHMN_20455: OKFHMN_20455 - DUF3561 domain-containing protein, at 3,982,529 to 3,982,852 _20455 OKFHMN_20460: ftsB - cell division protein FtsB, at 3,983,046 to 3,983,357 ftsB OKFHMN_20465: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 3,983,376 to 3,984,086 ispD Position (kb) 3982 3983Strain fitness (log2 ratio) -2 -1 0 1 2at 3981.577 kb on + strand, within cysNat 3981.578 kb on - strand, within cysNat 3981.580 kb on + strand, within cysNat 3981.664 kb on - strand, within cysNat 3981.664 kb on - strand, within cysNat 3981.732 kb on + strandat 3981.733 kb on - strandat 3981.760 kb on + strandat 3981.791 kb on - strandat 3981.872 kb on + strandat 3981.872 kb on + strandat 3981.873 kb on - strandat 3981.873 kb on - strandat 3981.939 kb on - strand, within cysCat 3981.959 kb on + strand, within cysCat 3981.972 kb on + strand, within cysCat 3982.028 kb on + strand, within cysCat 3982.129 kb on - strand, within cysCat 3982.313 kb on + strand, within cysCat 3982.313 kb on + strand, within cysCat 3982.313 kb on + strand, within cysCat 3982.315 kb on + strand, within cysCat 3982.315 kb on + strand, within cysCat 3982.317 kb on + strand, within cysCat 3982.317 kb on + strand, within cysCat 3982.318 kb on - strand, within cysCat 3982.416 kb on - strand, within cysCat 3982.416 kb on - strand, within cysCat 3982.515 kb on + strandat 3982.529 kb on - strandat 3982.535 kb on - strandat 3982.537 kb on + strandat 3982.549 kb on + strandat 3982.574 kb on - strand, within OKFHMN_20455at 3982.694 kb on + strand, within OKFHMN_20455at 3982.694 kb on + strand, within OKFHMN_20455at 3982.694 kb on + strand, within OKFHMN_20455at 3982.776 kb on + strand, within OKFHMN_20455at 3982.776 kb on + strand, within OKFHMN_20455at 3982.776 kb on + strand, within OKFHMN_20455at 3982.776 kb on + strand, within OKFHMN_20455at 3982.781 kb on + strand, within OKFHMN_20455at 3982.782 kb on - strand, within OKFHMN_20455at 3982.782 kb on - strand, within OKFHMN_20455at 3982.787 kb on - strand, within OKFHMN_20455at 3982.809 kb on + strand, within OKFHMN_20455at 3982.817 kb on - strand, within OKFHMN_20455at 3982.893 kb on - strandat 3982.896 kb on + strandat 3982.897 kb on - strandat 3982.908 kb on + strandat 3982.909 kb on - strandat 3982.930 kb on + strandat 3982.930 kb on + strandat 3982.930 kb on + strandat 3982.931 kb on - strandat 3982.931 kb on - strandat 3982.934 kb on + strandat 3982.935 kb on - strandat 3982.935 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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3,981,577 + cysN OKFHMN_20445 0.79 +1.0
3,981,578 - cysN OKFHMN_20445 0.79 +0.7
3,981,580 + cysN OKFHMN_20445 0.79 -2.1
3,981,664 - cysN OKFHMN_20445 0.85 +0.3
3,981,664 - cysN OKFHMN_20445 0.85 -1.2
3,981,732 + +0.8
3,981,733 - -0.9
3,981,760 + -1.3
3,981,791 - +0.6
3,981,872 + +0.1
3,981,872 + -0.3
3,981,873 - -0.2
3,981,873 - -1.0
3,981,939 - cysC OKFHMN_20450 0.11 -0.4
3,981,959 + cysC OKFHMN_20450 0.14 +1.4
3,981,972 + cysC OKFHMN_20450 0.16 -0.1
3,982,028 + cysC OKFHMN_20450 0.25 +0.8
3,982,129 - cysC OKFHMN_20450 0.42 -0.2
3,982,313 + cysC OKFHMN_20450 0.72 +0.0
3,982,313 + cysC OKFHMN_20450 0.72 +0.2
3,982,313 + cysC OKFHMN_20450 0.72 +0.2
3,982,315 + cysC OKFHMN_20450 0.73 +0.4
3,982,315 + cysC OKFHMN_20450 0.73 +0.0
3,982,317 + cysC OKFHMN_20450 0.73 +0.7
3,982,317 + cysC OKFHMN_20450 0.73 +1.8
3,982,318 - cysC OKFHMN_20450 0.73 -0.7
3,982,416 - cysC OKFHMN_20450 0.89 -0.8
3,982,416 - cysC OKFHMN_20450 0.89 -0.3
3,982,515 + -0.8
3,982,529 - +1.1
3,982,535 - -1.0
3,982,537 + -0.4
3,982,549 + -1.1
3,982,574 - OKFHMN_20455 0.14 -0.5
3,982,694 + OKFHMN_20455 0.51 +0.5
3,982,694 + OKFHMN_20455 0.51 -0.2
3,982,694 + OKFHMN_20455 0.51 -0.4
3,982,776 + OKFHMN_20455 0.76 +1.8
3,982,776 + OKFHMN_20455 0.76 +1.8
3,982,776 + OKFHMN_20455 0.76 +0.8
3,982,776 + OKFHMN_20455 0.76 -1.7
3,982,781 + OKFHMN_20455 0.78 +0.2
3,982,782 - OKFHMN_20455 0.78 +1.2
3,982,782 - OKFHMN_20455 0.78 +1.0
3,982,787 - OKFHMN_20455 0.80 +0.4
3,982,809 + OKFHMN_20455 0.86 -0.3
3,982,817 - OKFHMN_20455 0.89 +0.1
3,982,893 - -2.3
3,982,896 + -0.3
3,982,897 - -0.9
3,982,908 + +0.8
3,982,909 - +1.6
3,982,930 + -0.1
3,982,930 + -0.4
3,982,930 + +0.1
3,982,931 - -1.2
3,982,931 - -0.2
3,982,934 + -0.9
3,982,935 - -0.7
3,982,935 - +0.2

Or see this region's nucleotide sequence