Experiment: EV146
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysI and cysH are separated by 73 nucleotides cysH and hokG are separated by 263 nucleotides hokG and cas3 are separated by 191 nucleotides
OKFHMN_20375: cysI - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 3,966,172 to 3,967,884
cysI
OKFHMN_20380: cysH - phosphoadenosine phosphosulfate reductase, at 3,967,958 to 3,968,692
cysH
OKFHMN_20390: hokG - Putative protein HokG, at 3,968,956 to 3,969,108
hokG
OKFHMN_20395: cas3 - CRISPR-associated helicase/endonuclease Cas3, at 3,969,300 to 3,971,999
cas3
Position (kb)
3967
3968
3969 Strain fitness (log2 ratio)
-1
0
1
2 at 3966.997 kb on + strand, within cysI at 3966.997 kb on + strand, within cysI at 3967.096 kb on + strand, within cysI at 3967.097 kb on - strand, within cysI at 3967.128 kb on + strand, within cysI at 3967.207 kb on - strand, within cysI at 3967.234 kb on - strand, within cysI at 3967.584 kb on + strand, within cysI at 3967.723 kb on + strand at 3967.724 kb on - strand at 3967.958 kb on - strand at 3968.029 kb on + strand at 3968.100 kb on - strand, within cysH at 3968.236 kb on - strand, within cysH at 3968.268 kb on - strand, within cysH at 3968.349 kb on - strand, within cysH at 3968.526 kb on + strand, within cysH at 3968.528 kb on + strand, within cysH at 3968.558 kb on + strand, within cysH at 3968.605 kb on + strand, within cysH at 3968.803 kb on + strand at 3968.804 kb on - strand at 3968.804 kb on - strand at 3968.880 kb on + strand at 3968.928 kb on + strand at 3968.988 kb on - strand, within hokG at 3968.988 kb on - strand, within hokG at 3968.988 kb on - strand, within hokG at 3968.991 kb on - strand, within hokG at 3969.000 kb on + strand, within hokG at 3969.000 kb on + strand, within hokG at 3969.000 kb on + strand, within hokG at 3969.038 kb on + strand, within hokG at 3969.338 kb on + strand at 3969.594 kb on + strand, within cas3 at 3969.595 kb on - strand, within cas3
Per-strain Table
Position Strand Gene LocusTag Fraction EV146 remove 3,966,997 + cysI OKFHMN_20375 0.48 -0.5 3,966,997 + cysI OKFHMN_20375 0.48 -0.4 3,967,096 + cysI OKFHMN_20375 0.54 -1.6 3,967,097 - cysI OKFHMN_20375 0.54 -0.3 3,967,128 + cysI OKFHMN_20375 0.56 -0.5 3,967,207 - cysI OKFHMN_20375 0.60 -0.8 3,967,234 - cysI OKFHMN_20375 0.62 -0.6 3,967,584 + cysI OKFHMN_20375 0.82 +0.5 3,967,723 + -0.4 3,967,724 - -0.3 3,967,958 - -0.3 3,968,029 + +0.1 3,968,100 - cysH OKFHMN_20380 0.19 -0.6 3,968,236 - cysH OKFHMN_20380 0.38 +1.8 3,968,268 - cysH OKFHMN_20380 0.42 +0.3 3,968,349 - cysH OKFHMN_20380 0.53 +1.8 3,968,526 + cysH OKFHMN_20380 0.77 +0.8 3,968,528 + cysH OKFHMN_20380 0.78 +0.1 3,968,558 + cysH OKFHMN_20380 0.82 +0.7 3,968,605 + cysH OKFHMN_20380 0.88 -1.5 3,968,803 + -0.2 3,968,804 - +0.0 3,968,804 - +0.2 3,968,880 + +0.4 3,968,928 + -1.1 3,968,988 - hokG OKFHMN_20390 0.21 +0.2 3,968,988 - hokG OKFHMN_20390 0.21 +0.0 3,968,988 - hokG OKFHMN_20390 0.21 +0.4 3,968,991 - hokG OKFHMN_20390 0.23 -0.4 3,969,000 + hokG OKFHMN_20390 0.29 +0.2 3,969,000 + hokG OKFHMN_20390 0.29 -0.1 3,969,000 + hokG OKFHMN_20390 0.29 -0.8 3,969,038 + hokG OKFHMN_20390 0.54 +0.5 3,969,338 + +1.0 3,969,594 + cas3 OKFHMN_20395 0.11 +0.4 3,969,595 - cas3 OKFHMN_20395 0.11 +1.4
Or see this region's nucleotide sequence