Experiment: EV146
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpoA and rsmI are separated by 63 nucleotides rsmI and OKFHMN_18240 are separated by 42 nucleotides OKFHMN_18240 and tnp-IS3 overlap by 35 nucleotides
OKFHMN_18230: lpoA - penicillin-binding protein activator LpoA, at 3,538,462 to 3,540,498
lpoA
OKFHMN_18235: rsmI - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase, at 3,540,562 to 3,541,422
rsmI
OKFHMN_18240: OKFHMN_18240 - fimbrial protein, at 3,541,465 to 3,541,956
_18240
OKFHMN_18245: tnp-IS3 - IS3 family transposase, at 3,541,922 to 3,542,809
tnp-IS3
Position (kb)
3540
3541
3542 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3539.590 kb on + strand, within lpoA at 3539.591 kb on - strand, within lpoA at 3539.644 kb on - strand, within lpoA at 3539.901 kb on + strand, within lpoA at 3539.929 kb on - strand, within lpoA at 3539.929 kb on - strand, within lpoA at 3539.979 kb on + strand, within lpoA at 3540.026 kb on - strand, within lpoA at 3540.267 kb on + strand, within lpoA at 3540.299 kb on - strand at 3540.299 kb on - strand at 3540.319 kb on - strand at 3540.319 kb on - strand at 3540.319 kb on - strand at 3540.351 kb on - strand at 3540.384 kb on + strand at 3540.384 kb on + strand at 3540.384 kb on + strand at 3540.386 kb on + strand at 3540.386 kb on + strand at 3540.386 kb on + strand at 3540.386 kb on + strand at 3540.386 kb on + strand at 3540.387 kb on - strand at 3540.387 kb on - strand at 3540.387 kb on - strand at 3540.408 kb on + strand at 3540.409 kb on - strand at 3540.498 kb on + strand at 3540.499 kb on - strand at 3540.499 kb on - strand at 3540.605 kb on - strand at 3540.665 kb on + strand, within rsmI at 3540.665 kb on + strand, within rsmI at 3540.766 kb on - strand, within rsmI at 3540.869 kb on - strand, within rsmI at 3540.882 kb on + strand, within rsmI at 3540.937 kb on - strand, within rsmI at 3540.982 kb on + strand, within rsmI at 3541.028 kb on + strand, within rsmI at 3541.028 kb on + strand, within rsmI at 3541.089 kb on - strand, within rsmI at 3541.092 kb on + strand, within rsmI at 3541.093 kb on - strand, within rsmI at 3541.104 kb on + strand, within rsmI at 3541.104 kb on + strand, within rsmI at 3541.105 kb on - strand, within rsmI at 3541.118 kb on + strand, within rsmI at 3541.393 kb on + strand at 3541.393 kb on + strand at 3541.395 kb on + strand at 3541.395 kb on + strand at 3541.395 kb on + strand at 3541.395 kb on + strand at 3541.396 kb on - strand at 3541.396 kb on - strand at 3541.400 kb on - strand at 3541.400 kb on - strand at 3541.536 kb on + strand, within OKFHMN_18240 at 3541.536 kb on + strand, within OKFHMN_18240 at 3541.555 kb on - strand, within OKFHMN_18240 at 3541.593 kb on + strand, within OKFHMN_18240 at 3541.594 kb on - strand, within OKFHMN_18240 at 3541.612 kb on - strand, within OKFHMN_18240 at 3541.612 kb on - strand, within OKFHMN_18240 at 3541.642 kb on - strand, within OKFHMN_18240 at 3541.697 kb on + strand, within OKFHMN_18240 at 3541.697 kb on + strand, within OKFHMN_18240 at 3541.697 kb on + strand, within OKFHMN_18240 at 3541.698 kb on - strand, within OKFHMN_18240 at 3541.706 kb on - strand, within OKFHMN_18240 at 3541.776 kb on - strand, within OKFHMN_18240 at 3541.801 kb on - strand, within OKFHMN_18240 at 3541.866 kb on + strand, within OKFHMN_18240 at 3541.867 kb on - strand, within OKFHMN_18240
Per-strain Table
Position Strand Gene LocusTag Fraction EV146 remove 3,539,590 + lpoA OKFHMN_18230 0.55 +0.7 3,539,591 - lpoA OKFHMN_18230 0.55 -1.9 3,539,644 - lpoA OKFHMN_18230 0.58 -1.2 3,539,901 + lpoA OKFHMN_18230 0.71 +1.0 3,539,929 - lpoA OKFHMN_18230 0.72 -0.3 3,539,929 - lpoA OKFHMN_18230 0.72 -0.3 3,539,979 + lpoA OKFHMN_18230 0.74 -0.1 3,540,026 - lpoA OKFHMN_18230 0.77 -0.8 3,540,267 + lpoA OKFHMN_18230 0.89 -0.5 3,540,299 - -0.4 3,540,299 - -0.6 3,540,319 - -0.8 3,540,319 - -0.7 3,540,319 - -0.2 3,540,351 - -0.8 3,540,384 + -1.4 3,540,384 + -0.8 3,540,384 + -1.1 3,540,386 + +0.8 3,540,386 + +0.5 3,540,386 + +0.5 3,540,386 + -0.8 3,540,386 + -1.4 3,540,387 - +1.0 3,540,387 - -0.3 3,540,387 - -0.2 3,540,408 + -0.7 3,540,409 - -0.9 3,540,498 + +0.5 3,540,499 - +1.0 3,540,499 - -0.3 3,540,605 - -0.9 3,540,665 + rsmI OKFHMN_18235 0.12 -1.9 3,540,665 + rsmI OKFHMN_18235 0.12 -0.4 3,540,766 - rsmI OKFHMN_18235 0.24 -0.3 3,540,869 - rsmI OKFHMN_18235 0.36 +0.8 3,540,882 + rsmI OKFHMN_18235 0.37 -1.2 3,540,937 - rsmI OKFHMN_18235 0.44 +2.0 3,540,982 + rsmI OKFHMN_18235 0.49 +0.5 3,541,028 + rsmI OKFHMN_18235 0.54 -0.6 3,541,028 + rsmI OKFHMN_18235 0.54 +0.3 3,541,089 - rsmI OKFHMN_18235 0.61 -0.0 3,541,092 + rsmI OKFHMN_18235 0.62 -0.0 3,541,093 - rsmI OKFHMN_18235 0.62 -1.5 3,541,104 + rsmI OKFHMN_18235 0.63 -0.0 3,541,104 + rsmI OKFHMN_18235 0.63 -0.9 3,541,105 - rsmI OKFHMN_18235 0.63 +0.5 3,541,118 + rsmI OKFHMN_18235 0.65 +0.5 3,541,393 + -2.0 3,541,393 + +0.1 3,541,395 + +0.2 3,541,395 + +1.6 3,541,395 + +2.3 3,541,395 + +0.0 3,541,396 - -0.1 3,541,396 - +1.0 3,541,400 - +0.2 3,541,400 - -1.5 3,541,536 + OKFHMN_18240 0.14 -0.3 3,541,536 + OKFHMN_18240 0.14 -0.8 3,541,555 - OKFHMN_18240 0.18 +0.1 3,541,593 + OKFHMN_18240 0.26 +1.0 3,541,594 - OKFHMN_18240 0.26 -0.2 3,541,612 - OKFHMN_18240 0.30 -1.2 3,541,612 - OKFHMN_18240 0.30 -1.0 3,541,642 - OKFHMN_18240 0.36 -0.9 3,541,697 + OKFHMN_18240 0.47 -0.4 3,541,697 + OKFHMN_18240 0.47 +1.0 3,541,697 + OKFHMN_18240 0.47 +0.8 3,541,698 - OKFHMN_18240 0.47 +0.3 3,541,706 - OKFHMN_18240 0.49 +0.7 3,541,776 - OKFHMN_18240 0.63 +0.5 3,541,801 - OKFHMN_18240 0.68 +0.0 3,541,866 + OKFHMN_18240 0.82 -0.6 3,541,867 - OKFHMN_18240 0.82 +0.0
Or see this region's nucleotide sequence