Experiment: EV146
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sgcC and sgaB are separated by 76 nucleotides sgaB and ptsN overlap by 4 nucleotides ptsN and mngR are separated by 24 nucleotides mngR and nikE are separated by 198 nucleotides nikE and nikE are separated by 5 nucleotides
OKFHMN_16505: sgcC - PTS galactitol transporter subunit IIC, at 3,218,334 to 3,219,692
sgcC
OKFHMN_16510: sgaB - PTS sugar transporter subunit IIB, at 3,219,769 to 3,220,050
sgaB
OKFHMN_16515: ptsN - PTS suar transporter subunit IIA, at 3,220,047 to 3,220,520
ptsN
OKFHMN_16520: mngR - GntR family transcriptional regulator, at 3,220,545 to 3,221,291
mngR
OKFHMN_16525: nikE - nickel import ATP-binding protein NikE, at 3,221,490 to 3,221,891
nikE
OKFHMN_16530: nikE - nickel import ATP-binding protein NikE, at 3,221,897 to 3,222,703
nikE
Position (kb)
3220
3221
3222 Strain fitness (log2 ratio)
-1
0
1
2 at 3220.084 kb on - strand at 3220.128 kb on - strand, within ptsN at 3220.176 kb on - strand, within ptsN at 3220.225 kb on - strand, within ptsN at 3220.387 kb on - strand, within ptsN at 3220.490 kb on + strand at 3220.492 kb on + strand at 3220.542 kb on + strand at 3220.547 kb on - strand at 3220.600 kb on - strand at 3220.730 kb on - strand, within mngR at 3220.774 kb on - strand, within mngR at 3220.841 kb on + strand, within mngR at 3220.842 kb on - strand, within mngR at 3220.973 kb on + strand, within mngR at 3221.029 kb on - strand, within mngR at 3221.095 kb on - strand, within mngR at 3221.177 kb on - strand, within mngR at 3221.276 kb on + strand at 3221.276 kb on + strand at 3221.277 kb on - strand at 3221.348 kb on + strand at 3221.348 kb on + strand at 3221.349 kb on - strand at 3221.349 kb on - strand at 3221.351 kb on - strand at 3221.351 kb on - strand at 3221.353 kb on + strand at 3221.353 kb on + strand at 3221.354 kb on - strand at 3221.471 kb on - strand at 3221.484 kb on + strand at 3221.484 kb on + strand at 3221.485 kb on - strand at 3221.485 kb on - strand at 3221.485 kb on - strand at 3221.485 kb on - strand at 3221.634 kb on + strand, within nikE at 3221.634 kb on + strand, within nikE at 3221.635 kb on - strand, within nikE at 3221.635 kb on - strand, within nikE at 3221.681 kb on + strand, within nikE at 3221.713 kb on + strand, within nikE at 3221.713 kb on + strand, within nikE at 3221.719 kb on + strand, within nikE at 3221.720 kb on - strand, within nikE at 3221.720 kb on - strand, within nikE at 3221.816 kb on + strand, within nikE at 3221.816 kb on + strand, within nikE at 3221.817 kb on - strand, within nikE at 3221.856 kb on + strand at 3222.069 kb on - strand, within nikE at 3222.069 kb on - strand, within nikE at 3222.164 kb on - strand, within nikE at 3222.164 kb on - strand, within nikE at 3222.248 kb on + strand, within nikE
Per-strain Table
Position Strand Gene LocusTag Fraction EV146 remove 3,220,084 - +0.2 3,220,128 - ptsN OKFHMN_16515 0.17 +0.4 3,220,176 - ptsN OKFHMN_16515 0.27 -0.7 3,220,225 - ptsN OKFHMN_16515 0.38 +0.1 3,220,387 - ptsN OKFHMN_16515 0.72 +0.4 3,220,490 + -0.2 3,220,492 + +0.3 3,220,542 + -0.5 3,220,547 - -0.6 3,220,600 - -0.4 3,220,730 - mngR OKFHMN_16520 0.25 +0.5 3,220,774 - mngR OKFHMN_16520 0.31 -1.5 3,220,841 + mngR OKFHMN_16520 0.40 -0.5 3,220,842 - mngR OKFHMN_16520 0.40 -0.5 3,220,973 + mngR OKFHMN_16520 0.57 -0.3 3,221,029 - mngR OKFHMN_16520 0.65 +2.1 3,221,095 - mngR OKFHMN_16520 0.74 -1.5 3,221,177 - mngR OKFHMN_16520 0.85 -1.1 3,221,276 + -1.2 3,221,276 + +0.3 3,221,277 - +0.6 3,221,348 + +0.3 3,221,348 + -0.6 3,221,349 - -1.4 3,221,349 - +1.9 3,221,351 - +0.3 3,221,351 - -0.2 3,221,353 + -0.9 3,221,353 + -0.2 3,221,354 - +0.3 3,221,471 - +1.1 3,221,484 + -0.6 3,221,484 + -0.1 3,221,485 - -0.6 3,221,485 - -0.5 3,221,485 - -1.6 3,221,485 - +0.2 3,221,634 + nikE OKFHMN_16525 0.36 +0.3 3,221,634 + nikE OKFHMN_16525 0.36 -0.6 3,221,635 - nikE OKFHMN_16525 0.36 -0.8 3,221,635 - nikE OKFHMN_16525 0.36 +0.9 3,221,681 + nikE OKFHMN_16525 0.48 +0.3 3,221,713 + nikE OKFHMN_16525 0.55 -0.4 3,221,713 + nikE OKFHMN_16525 0.55 -0.4 3,221,719 + nikE OKFHMN_16525 0.57 -0.2 3,221,720 - nikE OKFHMN_16525 0.57 -0.4 3,221,720 - nikE OKFHMN_16525 0.57 -0.9 3,221,816 + nikE OKFHMN_16525 0.81 +0.0 3,221,816 + nikE OKFHMN_16525 0.81 -0.7 3,221,817 - nikE OKFHMN_16525 0.81 +0.2 3,221,856 + +0.9 3,222,069 - nikE OKFHMN_16530 0.21 +0.3 3,222,069 - nikE OKFHMN_16530 0.21 +0.5 3,222,164 - nikE OKFHMN_16530 0.33 +0.6 3,222,164 - nikE OKFHMN_16530 0.33 -0.2 3,222,248 + nikE OKFHMN_16530 0.43 -0.7
Or see this region's nucleotide sequence