Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15330

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgrlA and cesD are separated by 383 nucleotidescesD and escC are separated by 13 nucleotidesescC and sepD overlap by 1 nucleotidessepD and escJ are separated by 5 nucleotides OKFHMN_15320: grlA - type III secretion system LEE transcriptional regulator GrlA, at 2,960,899 to 2,961,312 grlA OKFHMN_15325: cesD - SycD/LcrH family type III secretion system chaperone CesD, at 2,961,696 to 2,962,151 cesD OKFHMN_15330: escC - type III secretion system LEE outer membrane ring protein EscC, at 2,962,165 to 2,963,703 escC OKFHMN_15335: sepD - type III secretion system LEE switch protein SepD, at 2,963,703 to 2,964,158 sepD OKFHMN_15340: escJ - type III secretion system LEE inner membrane ring protein EscJ, at 2,964,164 to 2,964,736 escJ Position (kb) 2962 2963 2964Strain fitness (log2 ratio) -2 -1 0 1at 2961.253 kb on - strand, within grlAat 2961.271 kb on - strandat 2961.468 kb on + strandat 2961.484 kb on + strandat 2961.726 kb on - strandat 2961.761 kb on + strand, within cesDat 2961.823 kb on + strand, within cesDat 2962.024 kb on + strand, within cesDat 2962.025 kb on - strand, within cesDat 2962.032 kb on - strand, within cesDat 2962.032 kb on - strand, within cesDat 2962.504 kb on - strand, within escCat 2962.771 kb on - strand, within escCat 2962.858 kb on - strand, within escCat 2963.127 kb on + strand, within escCat 2963.127 kb on + strand, within escCat 2963.446 kb on + strand, within escCat 2963.775 kb on + strand, within sepDat 2964.096 kb on + strand, within sepDat 2964.139 kb on - strandat 2964.158 kb on + strandat 2964.272 kb on + strand, within escJ

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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2,961,253 - grlA OKFHMN_15320 0.86 +0.2
2,961,271 - -0.2
2,961,468 + -0.2
2,961,484 + -0.4
2,961,726 - +0.6
2,961,761 + cesD OKFHMN_15325 0.14 +0.3
2,961,823 + cesD OKFHMN_15325 0.28 -0.0
2,962,024 + cesD OKFHMN_15325 0.72 +0.5
2,962,025 - cesD OKFHMN_15325 0.72 -1.9
2,962,032 - cesD OKFHMN_15325 0.74 +0.5
2,962,032 - cesD OKFHMN_15325 0.74 +0.9
2,962,504 - escC OKFHMN_15330 0.22 -0.2
2,962,771 - escC OKFHMN_15330 0.39 +0.4
2,962,858 - escC OKFHMN_15330 0.45 -0.9
2,963,127 + escC OKFHMN_15330 0.63 +0.3
2,963,127 + escC OKFHMN_15330 0.63 -0.1
2,963,446 + escC OKFHMN_15330 0.83 +0.0
2,963,775 + sepD OKFHMN_15335 0.16 +0.5
2,964,096 + sepD OKFHMN_15335 0.86 -0.0
2,964,139 - +0.1
2,964,158 + -0.4
2,964,272 + escJ OKFHMN_15340 0.19 -0.0

Or see this region's nucleotide sequence