Strain Fitness in Escherichia coli ECRC100 around OKFHMN_10020

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdkgB and Asp_trna are separated by 162 nucleotidesAsp_trna and 5S_rrna are separated by 54 nucleotides5S_rrna and 23S_rrna are separated by 94 nucleotides23S_rrna and Ala_trna are separated by 183 nucleotidesAla_trna and Ile_trna are separated by 42 nucleotidesIle_trna and 16S_rrna are separated by 68 nucleotides OKFHMN_10005: dkgB - 2,5-didehydrogluconate reductase DkgB, at 1,869,893 to 1,870,696 dkgB OKFHMN_10010: Asp_trna - tRNA-Asp(gtc), at 1,870,859 to 1,870,935 _trna OKFHMN_10015: 5S_rrna - 5S ribosomal RNA, at 1,870,990 to 1,871,105 _rrna OKFHMN_10020: 23S_rrna - 23S ribosomal RNA, at 1,871,200 to 1,874,102 _rrna OKFHMN_10030: Ala_trna - tRNA-Ala(tgc), at 1,874,286 to 1,874,361 _trna OKFHMN_10035: Ile_trna - tRNA-Ile(gat), at 1,874,404 to 1,874,480 _trna OKFHMN_10040: 16S_rrna - 16S ribosomal RNA, at 1,874,549 to 1,876,090 _rrna Position (kb) 1871 1872 1873 1874 1875Strain fitness (log2 ratio) -1 0 1at 1870.250 kb on - strand, within dkgBat 1870.366 kb on + strand, within dkgBat 1870.429 kb on - strand, within dkgBat 1870.460 kb on + strand, within dkgBat 1870.582 kb on - strand, within dkgBat 1870.696 kb on + strandat 1870.696 kb on + strandat 1870.856 kb on - strandat 1870.856 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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1,870,250 - dkgB OKFHMN_10005 0.44 +0.7
1,870,366 + dkgB OKFHMN_10005 0.59 -0.9
1,870,429 - dkgB OKFHMN_10005 0.67 -0.7
1,870,460 + dkgB OKFHMN_10005 0.71 -0.5
1,870,582 - dkgB OKFHMN_10005 0.86 -1.7
1,870,696 + -0.7
1,870,696 + -0.7
1,870,856 - -0.1
1,870,856 - -0.1

Or see this region's nucleotide sequence