Experiment: EV146
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiO and rlmF overlap by 4 nucleotides rlmF and mcbA are separated by 275 nucleotides mcbA and fiu are separated by 264 nucleotides
OKFHMN_06225: ybiO - mechanosensitive channel protein, at 1,103,343 to 1,105,568
ybiO
OKFHMN_06230: rlmF - 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF, at 1,105,565 to 1,106,491
rlmF
OKFHMN_06235: mcbA - DUF1471 domain-containing protein, at 1,106,767 to 1,107,027
mcbA
OKFHMN_06240: fiu - catecholate siderophore receptor Fiu, at 1,107,292 to 1,109,574
fiu
Position (kb)
1105
1106
1107 Strain fitness (log2 ratio)
-1
0
1
2 at 1104.872 kb on + strand, within ybiO at 1104.982 kb on + strand, within ybiO at 1105.025 kb on + strand, within ybiO at 1105.026 kb on - strand, within ybiO at 1105.263 kb on - strand, within ybiO at 1105.609 kb on + strand at 1105.888 kb on + strand, within rlmF at 1105.897 kb on + strand, within rlmF at 1105.898 kb on - strand, within rlmF at 1105.898 kb on - strand, within rlmF at 1106.033 kb on - strand, within rlmF at 1106.073 kb on - strand, within rlmF at 1106.123 kb on + strand, within rlmF at 1106.124 kb on - strand, within rlmF at 1106.124 kb on - strand, within rlmF at 1106.124 kb on - strand, within rlmF at 1106.182 kb on + strand, within rlmF at 1106.183 kb on - strand, within rlmF at 1106.233 kb on + strand, within rlmF at 1106.233 kb on + strand, within rlmF at 1106.233 kb on + strand, within rlmF at 1106.235 kb on + strand, within rlmF at 1106.235 kb on + strand, within rlmF at 1106.302 kb on - strand, within rlmF at 1106.384 kb on - strand, within rlmF at 1106.491 kb on + strand at 1106.492 kb on - strand at 1106.492 kb on - strand at 1106.517 kb on - strand at 1106.517 kb on - strand at 1106.546 kb on + strand at 1106.546 kb on + strand at 1106.547 kb on - strand at 1106.547 kb on - strand at 1106.723 kb on + strand at 1106.724 kb on - strand at 1106.748 kb on + strand at 1106.827 kb on + strand, within mcbA at 1106.828 kb on - strand, within mcbA at 1106.868 kb on - strand, within mcbA at 1106.965 kb on + strand, within mcbA at 1107.019 kb on + strand at 1107.020 kb on - strand at 1107.173 kb on - strand at 1107.203 kb on - strand at 1107.270 kb on - strand at 1107.270 kb on - strand at 1107.270 kb on - strand at 1107.357 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EV146 remove 1,104,872 + ybiO OKFHMN_06225 0.69 -0.3 1,104,982 + ybiO OKFHMN_06225 0.74 +1.7 1,105,025 + ybiO OKFHMN_06225 0.76 +0.0 1,105,026 - ybiO OKFHMN_06225 0.76 +0.7 1,105,263 - ybiO OKFHMN_06225 0.86 +0.1 1,105,609 + -0.7 1,105,888 + rlmF OKFHMN_06230 0.35 +1.1 1,105,897 + rlmF OKFHMN_06230 0.36 +0.1 1,105,898 - rlmF OKFHMN_06230 0.36 -0.6 1,105,898 - rlmF OKFHMN_06230 0.36 +0.3 1,106,033 - rlmF OKFHMN_06230 0.50 -0.3 1,106,073 - rlmF OKFHMN_06230 0.55 -0.4 1,106,123 + rlmF OKFHMN_06230 0.60 -0.7 1,106,124 - rlmF OKFHMN_06230 0.60 -0.0 1,106,124 - rlmF OKFHMN_06230 0.60 -0.0 1,106,124 - rlmF OKFHMN_06230 0.60 -0.3 1,106,182 + rlmF OKFHMN_06230 0.67 +0.1 1,106,183 - rlmF OKFHMN_06230 0.67 -0.9 1,106,233 + rlmF OKFHMN_06230 0.72 -0.1 1,106,233 + rlmF OKFHMN_06230 0.72 -0.7 1,106,233 + rlmF OKFHMN_06230 0.72 -1.2 1,106,235 + rlmF OKFHMN_06230 0.72 -0.6 1,106,235 + rlmF OKFHMN_06230 0.72 +2.1 1,106,302 - rlmF OKFHMN_06230 0.80 -0.8 1,106,384 - rlmF OKFHMN_06230 0.88 +1.7 1,106,491 + +0.6 1,106,492 - +0.3 1,106,492 - -0.0 1,106,517 - -0.9 1,106,517 - +0.3 1,106,546 + +0.7 1,106,546 + +0.1 1,106,547 - +0.2 1,106,547 - +0.2 1,106,723 + +0.5 1,106,724 - -0.4 1,106,748 + -0.3 1,106,827 + mcbA OKFHMN_06235 0.23 -1.5 1,106,828 - mcbA OKFHMN_06235 0.23 -0.9 1,106,868 - mcbA OKFHMN_06235 0.39 +0.1 1,106,965 + mcbA OKFHMN_06235 0.76 +0.6 1,107,019 + -0.8 1,107,020 - +0.3 1,107,173 - -0.5 1,107,203 - +0.5 1,107,270 - -0.5 1,107,270 - -0.5 1,107,270 - -0.0 1,107,357 + +0.1
Or see this region's nucleotide sequence