Strain Fitness in Escherichia coli ECRC100 around OKFHMN_06220

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntglnH and glnP are separated by 137 nucleotidesglnP and glnQ overlap by 4 nucleotidesglnQ and ybiO are separated by 116 nucleotides OKFHMN_06210: glnH - glutamine ABC transporter substrate-binding protein GlnH, at 1,100,964 to 1,101,710 glnH OKFHMN_06215: glnP - glutamine ABC transporter permease GlnP, at 1,101,848 to 1,102,507 glnP OKFHMN_06220: glnQ - glutamine ABC transporter ATP-binding protein GlnQ, at 1,102,504 to 1,103,226 glnQ OKFHMN_06225: ybiO - mechanosensitive channel protein, at 1,103,343 to 1,105,568 ybiO Position (kb) 1102 1103 1104Strain fitness (log2 ratio) -2 -1 0 1at 1101.588 kb on - strand, within glnHat 1101.667 kb on - strandat 1101.667 kb on - strandat 1101.667 kb on - strandat 1101.708 kb on + strandat 1101.711 kb on + strandat 1101.711 kb on + strandat 1101.718 kb on - strandat 1101.767 kb on + strandat 1101.800 kb on + strandat 1101.847 kb on + strandat 1101.848 kb on - strandat 1102.066 kb on + strand, within glnPat 1102.070 kb on + strand, within glnPat 1102.070 kb on + strand, within glnPat 1103.225 kb on - strandat 1103.303 kb on + strandat 1103.303 kb on + strandat 1103.303 kb on + strandat 1103.304 kb on - strandat 1103.306 kb on - strandat 1103.306 kb on - strandat 1103.306 kb on - strandat 1103.415 kb on - strandat 1103.702 kb on + strand, within ybiOat 1104.204 kb on - strand, within ybiOat 1104.223 kb on + strand, within ybiO

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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1,101,588 - glnH OKFHMN_06210 0.84 -1.5
1,101,667 - -2.5
1,101,667 - -0.4
1,101,667 - -0.2
1,101,708 + -1.2
1,101,711 + -0.5
1,101,711 + +1.1
1,101,718 - -0.1
1,101,767 + +0.8
1,101,800 + +0.5
1,101,847 + -0.1
1,101,848 - +0.4
1,102,066 + glnP OKFHMN_06215 0.33 +0.6
1,102,070 + glnP OKFHMN_06215 0.34 -0.4
1,102,070 + glnP OKFHMN_06215 0.34 -0.9
1,103,225 - +0.1
1,103,303 + -0.9
1,103,303 + +1.1
1,103,303 + +0.7
1,103,304 - -0.2
1,103,306 - -0.4
1,103,306 - +0.1
1,103,306 - +0.3
1,103,415 - -0.0
1,103,702 + ybiO OKFHMN_06225 0.16 +0.7
1,104,204 - ybiO OKFHMN_06225 0.39 -0.0
1,104,223 + ybiO OKFHMN_06225 0.40 +0.1

Or see this region's nucleotide sequence