Strain Fitness in Escherichia coli ECRC100 around OKFHMN_05865

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybjS and amiD overlap by 4 nucleotidesamiD and ybjQ overlap by 4 nucleotidesybjQ and OKFHMN_05875 are separated by 210 nucleotides OKFHMN_05860: ybjS - Uncharacterized protein YbjS, at 1,035,416 to 1,036,429 ybjS OKFHMN_05865: amiD - N-acetylmuramoyl-L-alanine amidase AmiD, at 1,036,426 to 1,037,256 amiD OKFHMN_05870: ybjQ - UPF0145 protein YbjQ, at 1,037,253 to 1,037,576 ybjQ OKFHMN_05875: OKFHMN_05875 - hypothetical protein, at 1,037,787 to 1,039,586 _05875 Position (kb) 1036 1037 1038Strain fitness (log2 ratio) -1 0 1at 1035.451 kb on - strandat 1035.596 kb on - strand, within ybjSat 1035.619 kb on + strand, within ybjSat 1035.705 kb on + strand, within ybjSat 1035.797 kb on - strand, within ybjSat 1035.872 kb on - strand, within ybjSat 1035.952 kb on + strand, within ybjSat 1035.971 kb on - strand, within ybjSat 1036.035 kb on - strand, within ybjSat 1036.109 kb on + strand, within ybjSat 1036.110 kb on - strand, within ybjSat 1036.110 kb on - strand, within ybjSat 1036.189 kb on + strand, within ybjSat 1036.219 kb on + strand, within ybjSat 1036.312 kb on + strand, within ybjSat 1036.313 kb on - strand, within ybjSat 1036.496 kb on + strandat 1036.497 kb on - strandat 1036.499 kb on - strandat 1036.800 kb on + strand, within amiDat 1036.822 kb on - strand, within amiDat 1037.043 kb on - strand, within amiDat 1037.086 kb on - strand, within amiDat 1037.106 kb on - strand, within amiDat 1037.218 kb on + strandat 1037.698 kb on + strandat 1037.699 kb on - strandat 1037.770 kb on - strandat 1037.858 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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1,035,451 - +1.1
1,035,596 - ybjS OKFHMN_05860 0.18 +0.3
1,035,619 + ybjS OKFHMN_05860 0.20 +0.7
1,035,705 + ybjS OKFHMN_05860 0.29 -0.6
1,035,797 - ybjS OKFHMN_05860 0.38 -0.1
1,035,872 - ybjS OKFHMN_05860 0.45 +0.2
1,035,952 + ybjS OKFHMN_05860 0.53 +1.5
1,035,971 - ybjS OKFHMN_05860 0.55 -0.1
1,036,035 - ybjS OKFHMN_05860 0.61 -0.7
1,036,109 + ybjS OKFHMN_05860 0.68 +0.9
1,036,110 - ybjS OKFHMN_05860 0.68 +0.3
1,036,110 - ybjS OKFHMN_05860 0.68 -0.1
1,036,189 + ybjS OKFHMN_05860 0.76 -0.3
1,036,219 + ybjS OKFHMN_05860 0.79 -0.2
1,036,312 + ybjS OKFHMN_05860 0.88 +0.5
1,036,313 - ybjS OKFHMN_05860 0.88 -1.3
1,036,496 + +0.7
1,036,497 - -0.3
1,036,499 - +0.5
1,036,800 + amiD OKFHMN_05865 0.45 +0.4
1,036,822 - amiD OKFHMN_05865 0.48 -0.3
1,037,043 - amiD OKFHMN_05865 0.74 +0.7
1,037,086 - amiD OKFHMN_05865 0.79 +1.0
1,037,106 - amiD OKFHMN_05865 0.82 -0.5
1,037,218 + +0.1
1,037,698 + -0.3
1,037,699 - +0.7
1,037,770 - -0.6
1,037,858 + +0.1

Or see this region's nucleotide sequence