Strain Fitness in Escherichia coli ECRC100 around OKFHMN_03690

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgA and flgM are separated by 75 nucleotidesflgM and flgN are separated by 4 nucleotidesflgN and murJ are separated by 39 nucleotides OKFHMN_03680: flgA - flagellar basal body P-ring formation protein FlgA, at 586,100 to 586,759 flgA OKFHMN_03685: flgM - anti-sigma-28 factor FlgM, at 586,835 to 587,128 flgM OKFHMN_03690: flgN - flagella biosynthesis chaperone FlgN, at 587,133 to 587,549 flgN OKFHMN_03695: murJ - murein biosynthesis integral membrane protein MurJ, at 587,589 to 589,124 murJ Position (kb) 587 588Strain fitness (log2 ratio) -1 0 1at 586.237 kb on + strand, within flgAat 586.243 kb on + strand, within flgAat 586.359 kb on + strand, within flgAat 586.366 kb on + strand, within flgAat 586.550 kb on - strand, within flgAat 586.710 kb on + strandat 586.789 kb on + strandat 587.132 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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586,237 + flgA OKFHMN_03680 0.21 -0.5
586,243 + flgA OKFHMN_03680 0.22 +1.1
586,359 + flgA OKFHMN_03680 0.39 +0.1
586,366 + flgA OKFHMN_03680 0.40 +0.8
586,550 - flgA OKFHMN_03680 0.68 +0.5
586,710 + +1.6
586,789 + +1.6
587,132 + -0.5

Or see this region's nucleotide sequence