Strain Fitness in Escherichia coli ECRC100 around OKFHMN_03630

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgL and flgK are separated by 11 nucleotidesflgK and flgJ are separated by 65 nucleotides OKFHMN_03625: flgL - flagellar hook-associated protein FlgL, at 576,014 to 576,967 flgL OKFHMN_03630: flgK - flagellar hook-associated protein FlgK, at 576,979 to 578,622 flgK OKFHMN_03635: flgJ - flagellar assembly peptidoglycan hydrolase FlgJ, at 578,688 to 579,629 flgJ Position (kb) 576 577 578 579Strain fitness (log2 ratio) -2 -1 0 1 2at 575.998 kb on - strandat 576.056 kb on - strandat 576.453 kb on - strand, within flgLat 576.453 kb on - strand, within flgLat 576.629 kb on - strand, within flgLat 576.728 kb on - strand, within flgLat 576.732 kb on - strand, within flgLat 577.345 kb on + strand, within flgKat 577.348 kb on + strand, within flgKat 577.735 kb on - strand, within flgKat 577.802 kb on - strand, within flgKat 577.950 kb on - strand, within flgKat 578.332 kb on - strand, within flgKat 578.448 kb on - strand, within flgKat 578.536 kb on - strandat 578.634 kb on - strandat 579.409 kb on + strand, within flgJat 579.409 kb on + strand, within flgJat 579.410 kb on - strand, within flgJ

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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575,998 - +2.2
576,056 - +0.9
576,453 - flgL OKFHMN_03625 0.46 +1.0
576,453 - flgL OKFHMN_03625 0.46 +0.6
576,629 - flgL OKFHMN_03625 0.64 -0.5
576,728 - flgL OKFHMN_03625 0.75 +0.2
576,732 - flgL OKFHMN_03625 0.75 -1.8
577,345 + flgK OKFHMN_03630 0.22 -0.0
577,348 + flgK OKFHMN_03630 0.22 -1.5
577,735 - flgK OKFHMN_03630 0.46 -0.2
577,802 - flgK OKFHMN_03630 0.50 -0.7
577,950 - flgK OKFHMN_03630 0.59 +0.2
578,332 - flgK OKFHMN_03630 0.82 -0.4
578,448 - flgK OKFHMN_03630 0.89 +0.8
578,536 - +0.6
578,634 - +0.9
579,409 + flgJ OKFHMN_03635 0.77 +0.1
579,409 + flgJ OKFHMN_03635 0.77 +0.3
579,410 - flgJ OKFHMN_03635 0.77 +1.6

Or see this region's nucleotide sequence